{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1342497600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"41708.jpg","Size":2838699,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"41708.zip","Size":23176806,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"41708.tif","Size":23300000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"DATE":["07\/24\/1977"],"Contributors":["Hans Ris"]},"BIOLOGICALPROCESS":[{"onto_name":"mitosis","onto_id":"GO:0007067"},{"onto_name":"mitotic metaphase","onto_id":"GO:0000089"},{"onto_name":"chromosome organization","onto_id":"GO:0051276"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"CHO"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"High Voltage (1MeV) EM image of a metaphase chromosome isolated from CHO cells using a non-aqueous technique, squashed in 1:1 acetic acid:ethanol and transferred to an EM grid. A chromatid pair occupies the center of the image. This micrograph was captured at a specimen tilt of 55 degrees. The accompanying image has a tilt of 45 degrees, creating a stereo pair that provides an oblique stereo view of the chromatids."},"ITEMTYPE":{"onto_name":"film","onto_id":"FBbi:00000303"},"CELLULARCOMPONENT":{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"},"RELATIONTOINTACTCELL":{"free_text":"squash"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"12259","TECHNICALDETAILS":{"free_text":"Cells were metaphase arrested with thymidine followed by colcemid, then exposed to a low ionic strength swelling medium (0.07M phosphate bufferin 50% D2O). Chromosomes were isolated by the non-aqueous hexylene glycol technique of Wray and Stubblefield, squashed in 1:1 acetic acid:ethanol,stained, and transferred to an EM grid.\nSee also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_id":"FBbi:00000622"},"DIMENSION":[{"Space":{"Image_size":2744,"Pixel_size":{"unit":"nanometers","value":1},"axis":"X"}},{"Space":{"Image_size":4222,"Pixel_size":{"unit":"nanometers","value":1},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Cricetulus griseus","onto_id":"NCBITaxon:10029"},"PREPARATION":{"onto_name":"chemically fixed tissue","onto_id":"FBbi:00000002"},"VISUALIZATIONMETHODS":{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},"CELLTYPE":{"free_text":"ovary"}}},"Citation":{"Title":"Hans Ris (2012) CIL:41708, Cricetulus griseus, ovary. CIL. Dataset","ARK":"ark:\/b7295\/w9cil41708","DOI":"doi:10.7295\/W9CIL41708"}}}