{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1298005200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"1311.tif","Size":42400000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"1311.zip","Size":42543536,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"1311.jpg","Size":6704138,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/www5.pbrc.hawaii.edu\/allen\/"}],"Contributors":["Richard Allen (University of Hawaii)"]},"BIOLOGICALPROCESS":[{"onto_name":"micronucleus organization","onto_id":"GO:0032125"},{"onto_name":"cytoplasm organization","onto_id":"GO:0007028"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"High resolution image of the interphase micronucleus with only a small amount of b-tubulin in its matrix or on its nuclear envelope. Segments of microtubules are scattered in the cytosol. Immunogold labeled for anti-b-tubulin. TEM taken on 7\/8\/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 12,000X. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of \u2013100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit\/goat and to colloidal gold-complexed goat-anti-mouse\/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The raw film was scanned with a  Nikon Coolscan 9000ED. This image is best used for quantitative analysis.  Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"micronucleus","onto_id":"GO:0031040"},{"onto_name":"cytoplasm","onto_id":"GO:0005737"},{"onto_name":"cytoplasmic microtubule","onto_id":"GO:0005881"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":[{"onto_name":"intrinsic mass distribution","onto_id":"FBbi:00000607"},{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"}],"GROUP_ID":"4091","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":3792,"Pixel_size":{"unit":"nanometers","value":1.25},"axis":"X"}},{"Space":{"Image_size":5582,"Pixel_size":{"unit":"nanometers","value":1.25},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Paramecium tetraurelia","onto_id":"NCBITaxon:5888"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"tissue in vitreous ice embedment","onto_id":"FBbi:00000089"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},{"free_text":"monoclonal antibody"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"gold","onto_id":"FBbi:00000202"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2011) CIL:1311, Paramecium tetraurelia, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil1311","DOI":"doi:10.7295\/W9CIL1311"}}}