Brandner, Dieter; Withers, Ginger (2021). CIL:10384, Rattus, multipolar neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0222TSR

Cultured hippocampal neurons after 23 days in vitro, immunostained for MAP2, a microtubule associated protein localized to dendrites (red), and PSD95, a scaffolding protein concentrated in the postsynaptic density of excitatory synapses, particularly in dendritic spines (green).

Detailed methods: Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for MAP2 (from S. Halpain) with d549 conjugated secondary, excitation, 555, emission, 568, Jackson Immunoresearch) and PSD95 (from NeuroMab, with Alexa488 conjugated secondary, excitation, 496, emission, 519, Invitrogen). Images were acquired with a Leica DMRA microscope with a 40X lens (HCX PL Fluotar, NA 0.75), Photometrics CoolSnap ES CCD camera and MetaMorph software. The merged image was generated by creating a MetaMorph stack file.

• 2021

• Brandner, Dieter
• Withers, Ginger

Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope; elastic scattering of photons
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 9921

• Rattus

• Dendrite
• Microtubule cytoskeleton
• Multipolar neuron
• Synapse part

• Cellular developmental process
• Dendrite development
• Establishment or maintenance of cell polarity
• Protein localization to synapse
• Synapse assembly

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10384

Doi: https://doi.org/10.6075/J0222TSR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10384

Creative Commons Attribution 4.0 International Public License

• UC Regents

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2022-08-12