{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1314849600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"37366.tif","Size":10800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"37366.jpg","Size":1330334,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"37366.zip","Size":10747526,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Clara Franzini-Armstrong","Kathryn Loesser"]},"BIOLOGICALPROCESS":[{"onto_name":"skeletal muscle contraction","onto_id":"GO:0003009"},{"onto_name":"mitochondrial proton-transporting ATP synthase complex assembly","onto_id":"GO:0033615"},{"onto_name":"sequestering of calcium ion","onto_id":"GO:0051208"},{"onto_name":"membrane depolarization","onto_id":"GO:0051899"}],"PROCESSINGHISTORY":{"free_text":"digitized negative film"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Longitudinal and cross sections showing ribbon like myofibrils regularly alternating with layers of mitochondria and SR\/T tubules.  Dyads are located in a grove of the mitochondrial surface.  Negatives aere scanned on nikon 8000 at 4000dpi\/6.3 micron scan step size, then reduced in size 4X for upload, for an effective pixel size of 25 micron.\n\nTechnical details:\nHeads and tail removed, thorax bisected and immersed in 3% glutaraldehyde buffered in 0.1 M cacodylate buffer (pH7.2). Stored at 4C for several days. Muscle dissected out. Rinsed 3Xin buffer, postfixed in 2% PsO4 in buffer, for 1.5 hrs; rinsed 3 times in water, en-block stained in saturated aqueous uranyl acetate, embedded in Spurr.  Thin sections stained in uranyl acetate and lead and examined in a Philips 410 Microscope.\n\nReference for the image:\nLoesser, K.E., Castellani, L. and Franzini-Armstrong, C. Disposition of junctional feet in muscles of invertebrates. J. Muscle Research Cell Motility 13:161-173, 199"},"ITEMTYPE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"CELLULARCOMPONENT":[{"onto_name":"myofibril","onto_id":"GO:0030016"},{"onto_name":"mitochondrion","onto_id":"GO:0005739"},{"onto_name":"sarcoplasmic reticulum","onto_id":"GO:0016529"},{"onto_name":"T-tubule","onto_id":"GO:0030315"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":2500,"axis":"X"}},{"Space":{"Image_size":2142,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"free_text":"damselfly"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"}],"VISUALIZATIONMETHODS":[{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":{"onto_name":"flight muscle cell","onto_id":"CL:0000196"}}},"Citation":{"Title":"Clara Franzini-Armstrong, Kathryn Loesser (2011) CIL:37366, damselfly, flight muscle cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil37366","DOI":"doi:10.7295\/W9CIL37366"}}}