{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1290402000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"561.jpg","Size":4959740,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"561.zip","Size":22919244,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"561.tif","Size":22800000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Chris Woodcock","Gayle Miller"]},"BIOLOGICALPROCESS":{"onto_name":"gamete generation","onto_id":"GO:0007276"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"At cyst formation in the 'giant' unicellular green alga Acetabularia, each cyst contains a single nucleus which subsequently undergoes several rounds of mitosis prior to gamete formation.  Initially the nuclei form a syncytium which separates into individual gametes. This section shows a stage in gametogenesis where the syncytium has just begun to divide into uninuclear gametes. See Woodcock and Miller, 1973. Ultrastructural features of the life cycle of Acetabularia I Gametogenesis. Proptoplasma 77, 313-319."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"chloroplast","onto_id":"GO:0009507"},{"onto_name":"plastid thylakoid membrane","onto_id":"GO:0055035"},{"onto_name":"chloroplast starch grain","onto_id":"GO:0009569"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"10296","TECHNICALDETAILS":{"free_text":"Cysts were fixed in glutaraldehyde, post-fixed in osmium tetroxide, epoxy embedded, and thin sections stained with aqueous uranyl acetate and lead citrate.  Grids were examined with a Philips 400 TEM operated at 80KV and images recorded on film."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"DIMENSION":[{"Space":{"Image_size":4040,"Pixel_size":{"unit":"nanometers","value":1.7},"axis":"X"}},{"Space":{"Image_size":2810,"Pixel_size":{"unit":"nanometers","value":1.7},"axis":"X"}}],"NCBIORGANISMALCLASSIFICATION":{"free_text":"Acetabularia mediterranea"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"tissue in plastic embedment","onto_id":"FBbi:00000016"}],"VISUALIZATIONMETHODS":[{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":{"onto_name":"gamete","onto_id":"CL:0000300"}}},"Citation":{"Title":"Chris Woodcock, Gayle Miller (2010) CIL:561, Acetabularia mediterranea, gamete. CIL. Dataset","ARK":"ark:\/b7295\/w9cil561","DOI":"doi:10.7295\/W9CIL561"}}}