{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1341979200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"41721.tif","Size":23500000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"41721.jpg","Size":4546029,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"41721.zip","Size":23388810,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"DATE":["06\/17\/1977"],"Contributors":["Hans Ris"]},"BIOLOGICALPROCESS":[{"onto_name":"mitosis","onto_id":"GO:0007067"},{"onto_name":"mitotic metaphase","onto_id":"GO:0000089"},{"onto_name":"chromosome organization","onto_id":"GO:0051276"}],"CELLLINE":{"free_text":"CHO"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"High Voltage EM image of CHO metaphase chromosomes from a 'thick' section of metaphase-arrested cells treated with D20 to stabilize microtubules. This image was recorded with a specimen tilt of 45 degrees.  The accompanying grouped image had a tilt of 55 degrees to create a stereo pair providing an oblique 3D view of the chromosomes."},"ITEMTYPE":{"onto_name":"film","onto_id":"FBbi:00000303"},"CELLULARCOMPONENT":{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"},"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"12041","TECHNICALDETAILS":{"free_text":"CHO cells at metaphase were treated with 50% D2O, fixed with glutaraldehyde, embedded in epoxy resin and 0.5 micrometer 'thick' sections stained with uranyl acetate and lead citrate for HVEM observation at 1 MeV. See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_id":"FBbi:00000622"},"DIMENSION":[{"Space":{"Image_size":2777,"Pixel_size":{"unit":"nanometers","value":1},"axis":"X"}},{"Space":{"Image_size":4210,"Pixel_size":{"unit":"nanometers","value":1},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Cricetulus griseus","onto_id":"NCBITaxon:10029"},"PREPARATION":{"onto_name":"chemically fixed tissue","onto_id":"FBbi:00000002"},"VISUALIZATIONMETHODS":{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},"CELLTYPE":{"free_text":"ovary"}}},"Citation":{"Title":"Hans Ris (2012) CIL:41721, Cricetulus griseus, ovary. CIL. Dataset","ARK":"ark:\/b7295\/w9cil41721","DOI":"doi:10.7295\/W9CIL41721"}}}