Microscopy product ID: 4096
Segmentation
| Description | Manual segmentation of the individual cristae and inner and outer mitochondrial membranes using Xvoxtrace 2.18. Contours were traced every other section on even numbered planes. |
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| Description | Zip file containing the .trace file generated by Xvoxtrace (RudyD.trace) containing the manual traces and the subvolume in Analyze 7.5 format (RudyD_sub1.img/.hdr files) used for tracing. Surfaces generated from the contours for each object are included in Synu format (*.synu). Note that both Xvoxtrace and synu can now be read using Jinx, available from the CCDB download page. |
Reconstruction
| Description | Computed slice through an electron tomographic volume of mitochondriaisolated from mouse liver and treated with the serine protease inhibitor MG132. |
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| Description | Zip file (RudyD_vol.zip) containing the tomographic reconstruction in Analyze 7.5 format: RudyD_sub.img/hdr. This file contains the full sized reconstruction, despite its name. A subvolume of this was used for segmentation (see image map file). This subvolume is available with the segmentation file download. |
Animation
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| Description | Animation through a portion of the tomographic volume of isolated mitochondria treated with the serine protease inhibitor MG132. The animation was downsampled for ease of viewing. (RudyD_vol.avi) |
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- Cite This Work
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Yamaguchi, Ryuji; Newmeyer, Don; Lartigue, Lydia; Perkins, Guy A.; Scott, Ray T.; Dixit, Amruta; (2017). Microscopy product ID: 4096. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0959HCH
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
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Male
Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000EX IVEM
- Series
- Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Related Resources
- Yamaguchi R, Lartigue L, Perkins G, Scott RT, Dixit A, Kushnareva Y, Kuwana T, Ellisman MH, Newmeyer DD (2008). Opa1-mediated cristae opening is Bax/Bak and BH3 dependent, required for apoptosis, and independent of Bak oligomerization. Molecular Cell, 31(4):557-569. PubMed: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2636708; Molecular Cell. https://doi.org/10.1016/j.molcel.2008.07.010
- Microscopy product 4096 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB4096
Primary associated publication
Other version
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Creative Commons Attribution 4.0 International Public License
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- UC Regents
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Under copyright (US)
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22