{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1295413200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"9823.jpg","Size":1325529,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"9823.tif","Size":8100000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"9823.zip","Size":16128474,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen"]},"BIOLOGICALPROCESS":[{"onto_name":"macronucleus organization","onto_id":"GO:0032124"},{"onto_name":"cortical cytoskeleton organization","onto_id":"GO:0030865"},{"onto_name":"cytoplasm organization","onto_id":"GO:0007028"},{"onto_name":"oral apparatus organization","onto_id":"GO:0032122"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop"}],"CELLLINE":{"free_text":"Cambridge Culture Collection, Cambridge, England, # LB1657\/1"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Opercularia is named for a ciliated flap (operculum) that is adoral to the opening to the oral region. However, this operculum is pulled back into the body when the myonemes are fully contracted as in this figure. The cilia bordering the operculum arise from the perioral kinetids. TEM taken on 6\/13\/69 by R. Allen with Philips 300 operating at 60kV. Neg. 4,270X. Bar = 1µm.  A print of the negative was scanned and processed in Photoshop. This image is suitable for qualitative analysis. There is a high resolution version of this image in the library (CIL:39170) which is available for quantitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"macronucleus","onto_id":"GO:0031039"},{"onto_name":"oral apparatus","onto_id":"GO:0031912"},{"onto_name":"mitochondrion","onto_id":"GO:0005739"},{"onto_name":"cell cortex","onto_id":"GO:0005938"},{"onto_name":"cytoplasm","onto_id":"GO:0005737"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"10061","TECHNICALDETAILS":{"free_text":"Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":2829,"axis":"X"}},{"Space":{"Image_size":2823,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Opercularia [NCBITaxon:168247]","onto_id":"NCBITaxon:168247"},{"free_text":"Opercularia coarctata"}],"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (2011) CIL:9823, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil9823","DOI":"doi:10.7295\/W9CIL9823"}}}