{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1307073600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"34930.tif","Size":1600000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"34930.jpg","Size":131540,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"34930.zip","Size":3158710,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol. 1999 May 31;145(5):1009-26."],"Contributors":["Tatyana M. Svitkina","Gary G. Borisy"],"PUBMED":["10352018"]},"BIOLOGICALPROCESS":[{"free_text":"branching of actin filaments"},{"free_text":"cytochalasin D treatment"},{"free_text":"linking of actin filaments"}],"PROCESSINGHISTORY":{"free_text":"pseudocolored"},"PARAMETERIMAGED":{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"},"IMAGEDESCRIPTION":{"free_text":"Localization of cross-linking proteins in fibroblast cytoskeleton. Immuno-EM of the cell interior at cell edge  (CIL 34926) of cytochalasin D-treated Xenopus fibroblasts stained with α-actinin primary antibody and 18-nm gold-conjugated secondary antibody. Gold particles (yellow) reveal α-actinin at filament crossovers in the cell interior.  A fluorescence image of  fibroblast lamellipodia double stained with TRITC-phalloidin (red) and either  α-actinin (green) is available at CIL 34924. The image corresponds to Figure 5f from J Cell Biol. 1999 May 31;145(5):1009-26."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"},{"free_text":"alpha actinin"},{"free_text":"cell interior"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"GROUP_ID":"9875","MOLECULARFUNCTION":{"onto_name":"actin filament-based process","onto_id":"GO:0030029"},"TECHNICALDETAILS":{"free_text":"Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998)."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":780,"Pixel_size":{"unit":"nanometers","value":2.75},"axis":"X"}},{"Space":{"Image_size":661,"Pixel_size":{"unit":"nanometers","value":2.75},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Xenopus laevis","onto_id":"NCBITaxon:8355"},"PREPARATION":[{"onto_name":"permeabilized tissue","onto_id":"FBbi:00000093"},{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"free_text":"phalloidin"},{"onto_name":"methanol fixed tissue","onto_id":"FBbi:00000007"}],"VISUALIZATIONMETHODS":[{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},{"free_text":"platinum replica"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"free_text":"immuno-gold"}],"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"}}},"Citation":{"Title":"Tatyana M. Svitkina, Gary G. Borisy (2011) CIL:34930, Xenopus laevis, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil34930","DOI":"doi:10.7295\/W9CIL34930"}}}