3998
2004-05-12 00:00:00.0
Patrick Viollier
Tom Deerinck
purpose
Tomographic reconstruction of caulobacter
A .mpg animation of the aligned tilt series acquired from an IVEM of a Caulobacter crescentus sample, strain Snc04
/telescience/home/CCDB_DATA_USER.portal/P1220/Experiment_3374/Subject_233/Tissue_299/Microscopy_3998/cb012_tilt.mpg
The zero degree tilt view of the raw data acquired from an IVEM of a Caulobacter crescentus sample, strain Snc04
<![CDATA[http://ccdb.ucsd.edu/sand/main?event=rd&type=512r&mpid=3998]]>
A .tar file containing all necessary IMOD processing files for a tomographic reconstruction of Caulobacter crescentus. Raw MRC tilt data is included in this
/telescience/home/CCDB_DATA_USER.portal/P1220/Experiment_3374/Subject_233/Tissue_299/Microscopy_3998/cb012_img.tar
400 keV
0
66 degrees
-52 degrees
singlet_desc
2 degrees
2007-12-12 00:00:00.0
3998
cb012
JEOL 4000 #1
IVEM
SINGLE TILT
.5 nm/pixels
4096
.5 nm/pixels
4096
Patrick H. Viollier
Tom Deerinck
Mason Mackey
John Crum
Mark Ellisman
Department of Energy
Lucy Shapiro
Harley McAdams
Caulobacter
2003-07-01 00:00:00.0
P1220
A .mpg animation file of the slice by slice view of the backprojected volume.
/telescience/home/CCDB_DATA_USER.portal/P1220/Experiment_3374/Subject_233/Tissue_299/Microscopy_3998/cb012_slicebyslice.mpg
A slice by slice view from the backprojected volume of Caulobacter crescentus
<![CDATA[http://ccdb.ucsd.edu/sand/main?event=rd&type=512v&mpid=3998]]>
A .tar file containing 2 MRC format backprojected volumes of Caulobacter crescentus. One is the uncropped version of the volume with XY slices flipped with XZ slices. The other is a smaller cropped and flipped version of the volume.
Both volumes have been binned by 2 from the original resolution
/telescience/home/CCDB_DATA_USER.portal/P1220/Experiment_3374/Subject_233/Tissue_299/Microscopy_3998/cb012_vol.tar
A single MRC file of the volume that has been cropped and flipped after backprojecting.
/telescience/home/CCDB_DATA_USER.portal/P1220/Experiment_3374/Subject_233/Tissue_299/Microscopy_3998/cb012.rec
MRC
1172,2046,184
.001,.001,.0005
Caulobacter crescentus
1) Induced for 1 hour at 30 degrees2) Labeled for 2 hours at 30 degrees (in presence of 1.8 micromolar ReAsH-EDT2)3) Post fixed with _0.01%_ Acrolein and 2.5% of Glut. in 0.01M Sodium Cocadalyte buffer.4) HPF5) FS by using Epoxy resin as fixative: 20% of the complete Araldite/Epon formulation in acetone. Infiltration and embedding in 100% of resin complex.6) 0.5um sections were cut and collected on gold grids. Sections were post stained with 4% of Osmium followed by Sato lead and finally 10nm was applied on both sides of sections.
HPF / FS epoxy embedded, no photoconversion
N/A
male
Caulobacter crescentus (Snc04)
Caulobacter
Snc04
N/A
.5 um
N/A
None