{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1330318800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"39943.jpg","Size":181199,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"39943.tif","Size":3400000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"CCDB 1085","Href":"http:\/\/ccdb.ucsd.edu\/sand\/main?event=displayAll&mpid=1085"}],"Contributors":["Eric A. Bushong","Maryann E. Martone","Mark H. Ellisman"],"PUBMED":["PMID:15036382"]},"BIOLOGICALPROCESS":[{"onto_name":"forebrain astrocyte development","onto_id":"GO:0021897"},{"onto_name":"cell projection organization","onto_id":"GO:0030030"}],"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of a 4 week old rat reveals highly ramified spongiform processes. Astrocytes were filled with Lucifer Yellow.  Optical sections were generated with a single photon confocal microscope, and compiled here as a single maximum projection image.  This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"cell projection cytoplasm","onto_id":"GO:0032838"},{"onto_name":"cell body","onto_id":"GO:0044297"}],"SOURCEOFCONTRAST":{"onto_name":"intracellular filling","onto_id":"FBbi:00000088"},"RELATIONTOINTACTCELL":{"onto_name":"vibratome-sectioned tissue","onto_id":"FBbi:00000030"},"GROUP_ID":"11048","TECHNICALDETAILS":{"free_text":"Male Sprague Dawley rats, 4 weeks of age, were anesthetized and perfused transcardially with with Ringer\u2019s solution (37°C), followed by 4% paraformaldehyde (PFA) in PBS (37°C, pH 7.4).  Tissue was sectioned (100 µm thick) using a vibratome, and injected  with a BX50WI microscope (Olympus, Melville, NY) and, using infrared differential interference contrast videomicroscopy, cells with a small diameter soma (approx. 7\u201311µm) in the stratum\nradiatum of CA1 were located and impaled with glass micropipettes filled with 5% aq. Lucifer Yellow. The astrocytes were filled using 0.5 Hz pulses of current until all of the processes were brightly fluorescent. After several astrocytes were filled, a slice was placed in the 4% PFA overnight at 4°C. Slices were then imaged after coverslipping in Gelvatol.  Confocal images were acquired with a BioRad Radiance2000 (Hercules, CA) attached to a Nikon E600FN (Kanagawa, Japan) microscope equipped with a Nikon (Tokyo, Japan) 60x oil objective (NA 1.4). Additional details are provided in Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86."},"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":1052,"axis":"X"}},{"Space":{"Image_size":1052,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Rattus norvegicus","onto_id":"NCBITaxon:10116"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":{"onto_name":"lucifer yellow","onto_id":"FBbi:00000057"},"CELLTYPE":[{"onto_name":"astrocyte","onto_id":"CL:0000127"},{"onto_name":"astrocyte of the hippocampus","onto_id":"CL:0002604"}]}},"Citation":{"Title":"Eric A. Bushong, Maryann E. Martone, Mark H. Ellisman (2012) CIL:39943, Rattus norvegicus, astrocyte, astrocyte of the hippocampus. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39943","DOI":"doi:10.7295\/W9CIL39943"}}}