{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1292734800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"10102.tif","Size":4200000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"10102.jpg","Size":57462,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"10102.zip","Size":4133755,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Allen Liu","Sandra L. Schmid"]},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"ARPE-19","onto_id":"MCC:0000038"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Cultured retinal pigment epithelial cells immunofluorescently labeled for clathrin (green) and nucleus (blue).  The cells were fixed in 2% PFA and 0.5% Triton X-100 for 2 minutes followed by post-fixation 4% PFA.  Clathrin was detected with X22 primary antibody and secondary FITC antibody.  The nucleus was detected with  DAPI staining. Images were collected on an  Olympus IX-71 epifluorescence microscope using a 100X 1.4 NA objective with 4.500ms exposure for clathrin and 50ms exposure for DAPI (67nm\/pixel)."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"clathrin coat","onto_id":"GO:0030118"},{"onto_name":"nucleus","onto_id":"GO:0005634"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":1344,"Pixel_size":{"unit":"nanometers","value":67},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"nanometers","value":67},"axis":"Y"}},{"Wavelength":{"unit":"nanometers","value":"350, 488"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"permeabilized tissue","onto_id":"FBbi:00000093"}],"VISUALIZATIONMETHODS":[{"onto_name":"4',6-diamidino-2-phenylindole (DAPI)","onto_id":"FBbi:00000056"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"}],"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Allen Liu, Sandra L. Schmid (2010) CIL:10102, Homo sapiens, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil10102","DOI":"doi:10.7295\/W9CIL10102"}}}