CIL:9922, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:9922, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0XD10VJ
- Description
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An overview of the peristome of Opercularia coarctata. The corrugated wall, the equivalent to the ribbed wall of Paramecium, contains tubular projections of alveoli and cross sections of two ribbons of microtubules, one of 2 microtubules and one of 4 microtubules. The ends of these ribbons, at their origins, are covered by a myoneme which, in turn, is accompanied by a layer of ER. The microtubules will extend along the wall of the peristome and some will pass beyond the cytostome to form the lamellae of the cytopharynx. Vesicles bind to the lamellae and provide membrane for the nascent food vacuole. Oral polykinetids, myoneme, endoplasmic reticulum, contractile vacuole, microtubular ribbons, pellicular pores, and Golgi complex can be seen. TEM taken on 6/10/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:7335) is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Date Issued
- 2021
- Researcher
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: film; print of negative scanned for photoshop - Series
- Scientific Names
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- No linguistic content; Not applicable
- Identifier
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Samplenumber: 9922
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9922
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12