{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1290315600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"9695.tif","Size":7700000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"9695.zip","Size":15209726,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"9695.jpg","Size":2123736,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen"]},"BIOLOGICALPROCESS":[{"free_text":"ionic transport"},{"onto_name":"ciliary or flagellar motility","onto_id":"GO:0001539"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop."}],"CELLLINE":{"free_text":"Cambridge Culture Collection, Cambridge, England, catalog # LB1656\/1"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Longitudinal section of four kinetal rows of Coleps showing transverse sections of cilia and basal bodies. Parasomal sacs\/clathrin coated pits penetrate through the plasma membrane into the cytoplasm adjacent to each basal body. An accumulation of early endosome-associated vesicles are near each parasomal sac. Cortical tubular mitochondria are evident. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5\/23\/69 by R. Allen with Philips 300 operating at 60kV. Neg. 6,370X. Bar = 1µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution is available at (CIL:38827) and can be used in quantitative analysis. Additional information is available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"plasma membrane","onto_id":"GO:0005886"},{"free_text":"parasomal sacs or clathrin coated pits"},{"free_text":"alveolar membranes"},{"free_text":"cortical mitochondria"},{"onto_name":"cilium","onto_id":"GO:0005929"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"9806","MOLECULARFUNCTION":{"free_text":"cellular homeostasis"},"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":3000,"axis":"X"}},{"Space":{"Image_size":2530,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Coleps hirtus","onto_id":"NCBITaxon:60000"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (2010) CIL:9695, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil9695","DOI":"doi:10.7295\/W9CIL9695"}}}