{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1299819600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"13906.jpg","Size":138708,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"13906.tif","Size":12600000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"13906.zip","Size":12600187,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/4055\/"}],"PUBLISHED":["J Cell Biol 192: 615-629"],"Contributors":["Eugenia Morselli","Guillermo Mariño","Martin v. Bennetzen","Tobias Eisenberg","Evgenia Megalou","Sabrina Schroeder","Sandra Cabrera","Paule Bénit","Pierre Rustin","Alfredo Criollo","Oliver Kepp","Lorenzo Galluzzi","Shensi Shen","Shoaib Ahmad Malik","Maria Chiara Maiuri","Yoshiyuki Horio","Carlos López-Otín","Jens S. Andersen","Nektarios Tavernarakis","Frank Madeo","Guido Kroemer"],"PUBMED":["21339330"]},"BIOLOGICALPROCESS":{"onto_name":"autophagy","onto_id":"GO:0006914"},"PROCESSINGHISTORY":{"free_text":"brightness and contrast adjusted"},"CELLLINE":{"onto_name":"HCT 116","onto_id":"MCC:0000181"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Human colon carcinoma HCT 116 cells were transfected with an siRNA specific for SIRT1 and then retransfected with a GFP-LC3-encoding plasmid, cultured in complete medium for 24 h and left untreated for 4 h as a control for resveratrol or spermidine treatment. For fluorescence microscopy determinations, cells cultured on coverslips were fixed in paraformaldehyde (4% wt\/vol) for 15 min at RT, washed three times in PBS, and mounted with mounting medium (Vectashield). Confocal fluorescent images were captured using a confocal fluorescence microscope (TCS SP2; Leica) fitted with an Apochromat 63× 1.3 NA immersion objective. Images were acquired with a camera (DFC 350 FX 1.8.0; Leica) using LAS AF software (Leica) and processed with Photoshop (CS2; Adobe) software.  Specifically, picture processing involved cropping of representative areas and linear adjustments of contrast and brightness and was performed using Photoshop (with equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 1A, bottom left panel, in Morselli et al. J Cell Biol 192: 615-629"},"ITEMTYPE":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"}],"CELLULARCOMPONENT":{"onto_name":"autophagic vacuole","onto_id":"GO:0005776"},"RELATIONTOINTACTCELL":[{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},{"free_text":"cultured cells in vitro"}],"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"5405","DATAQUALIFICATION":{"free_text":"PROCESSED;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":2048,"axis":"X"}},{"Space":{"Image_size":2048,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"},"CELLTYPE":{"onto_name":"permanent cell line cell","onto_id":"CL:0000002"}}},"Citation":{"Title":"Eugenia Morselli, Guillermo Mariño, Martin v. Bennetzen, Tobias Eisenberg, Evgenia Megalou, Sabrina Schroeder, Sandra Cabrera, Paule Bénit, Pierre Rustin, Alfredo Criollo, Oliver Kepp, Lorenzo Galluzzi, Shensi Shen, Shoaib Ahmad Malik, Maria Chiara Maiuri, Yoshiyuki Horio, Carlos López-Otín, Jens S. Andersen, Nektarios Tavernarakis, Frank Madeo, Guido Kroemer (2011) CIL:13906, Homo sapiens, permanent cell line cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13906","DOI":"doi:10.7295\/W9CIL13906"}}}