{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1301284800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"13560.zip","Size":8402124,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"13560.tif","Size":8500000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13560.jpg","Size":174208,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/2860\/"}],"PUBLISHED":["J Cell Biol. 189: 843-858. 2010."],"Contributors":["David J. Gill","Joanne Chia","Jamie Senewiratne","Frederic Bard"],"PUBMED":["PMID: 20498016"]},"BIOLOGICALPROCESS":[{"onto_name":"Golgi organization","onto_id":"GO:0007030"},{"onto_name":"protein folding in endoplasmic reticulum","onto_id":"GO:0034975"}],"CELLLINE":{"onto_name":"HeLa","onto_id":"MCC:0000219"},"PARAMETERIMAGED":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"IMAGEDESCRIPTION":{"free_text":"An ER-trapped GalNac-T activity reporter (Muc-PTS; fused to GFP) (green) is targeted to the ER and not the Golgi, marked by Giantin (red) in HeLa cells cotransfected with inactive Src (SrcK-mCherry, containing K295M mutation) (gray). Cells were fixed for 10 min (4% paraformaldehyde) and permeabilized (0.2% Triton X-100). Primary antibody staining followed the manufacturer\u2019s instructions. Cells were subsequently stained for 15\u201330 min with secondary Alexa 647 for anti-Giantin and Hoechst (blue). Cells were mounted onto glass slides using FluorSave (Merck) and imaged at room temperature using an inverted FluoView confocal microscope (model IX81; Olympus) with fluorescence excitation at  488 nm, 561 nm and 633 nm and either a 60x objective (U Plan Super Apochromatic [UPLSAPO]; NA 1.35) or 100x objective (UPLSAPO; NA 1.40) using Immersol oil. Microscope coupled with a CCD camera (model FVII). Images were acquired and processed using Olympus FV10-ASW software.  Image corresponds to Fig 5C in J Cell Biol. 189: 843-858. 2010. Images in Fig 5 include CIL#s 13559, 13560, 13561, 13562."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"Golgi stack","onto_id":"GO:0005795"},{"onto_name":"integral to membrane","onto_id":"GO:0016021"},{"onto_name":"endoplasmic reticulum","onto_id":"GO:0005783"},{"onto_name":"nucleus","onto_id":"GO:0005634"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"}],"GROUP_ID":"6490","MOLECULARFUNCTION":[{"onto_name":"protein disulfide isomerase activity","onto_id":"GO:0003756"},{"onto_name":"N-acetylgalactosamine binding","onto_id":"GO:0046871"}],"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.103},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.103},"axis":"Y"}},{"Space":{"Image_size":1,"Pixel_size":{"unit":"microns","value":3.09},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 647","onto_id":"FBbi:00000447"},{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"onto_name":"mCherryFP","onto_id":"FBbi:00000525"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"free_text":"Hoechst"}],"CELLTYPE":[{"onto_name":"epithelial cell","onto_id":"CL:0000066"},{"free_text":"cervical carcinoma"}]}},"Citation":{"Title":"David J. Gill, Joanne Chia, Jamie Senewiratne, Frederic Bard (2011) CIL:13560, Homo sapiens, epithelial cell, cervical carcinoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13560","DOI":"doi:10.7295\/W9CIL13560"}}}