{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1298005200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"12081.jpg","Size":8547621,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"12081.zip","Size":65197690,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"12081.tif","Size":65300000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/www5.pbrc.hawaii.edu\/allen\/"}],"Contributors":["Richard Allen (University of Hawaii)"]},"BIOLOGICALPROCESS":[{"onto_name":"microtubule cytoskeleton organization","onto_id":"GO:0000226"},{"onto_name":"oral apparatus organization","onto_id":"GO:0032122"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"High resolution view of the quadrulus membranelle (4 rows of cilia) in the buccal cavity of Paramecium tetraurelia labelled for tubulin. Microtubules of the axonemes of the cilia and the triplet fibers of the basal bodies are labeled. Ribbons of microtubules arise from the side of the quadrulus next to the cytopharynx and single microtubules are liberally scattered about in this zone. The \u201Csperm-shaped\u201D structures are early endosomes with clathrin coated pits forming the \"heads.\" TEM taken on 7\/5\/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"cell cortex","onto_id":"GO:0005938"},{"onto_name":"cortical microtubule cytoskeleton","onto_id":"GO:0030981"},{"onto_name":"cortical microtubule","onto_id":"GO:0055028"},{"onto_name":"oral apparatus","onto_id":"GO:0031912"},{"onto_name":"axonemal microtubule","onto_id":"GO:0005879"},{"onto_name":"microtubule basal body","onto_id":"GO:0005932"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":[{"onto_name":"intrinsic mass distribution","onto_id":"FBbi:00000607"},{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"}],"GROUP_ID":"9634","TECHNICALDETAILS":{"free_text":"Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of \u2013100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit\/goat and to colloidal gold-complexed goat-anti-mouse\/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The raw film was scanned with a  Nikon Coolscan 9000ED. This image is best used for quantitative analysis.  Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":6079,"Pixel_size":{"unit":"nanometers","value":1.4},"axis":"X"}},{"Space":{"Image_size":5362,"Pixel_size":{"unit":"nanometers","value":1.4},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Paramecium tetraurelia","onto_id":"NCBITaxon:5888"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"tissue in vitreous ice embedment","onto_id":"FBbi:00000089"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},{"free_text":"monoclonal antibody"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"gold","onto_id":"FBbi:00000202"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2011) CIL:12081, Paramecium tetraurelia, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil12081","DOI":"doi:10.7295\/W9CIL12081"}}}