{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1313726400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"36300.tif","Size":3400000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"36300.jpg","Size":211997,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"36300.zip","Size":1418537,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"CCDB ID 1005","Href":"http:\/\/ccdb.ucsd.edu\/sand\/main?mpid=1005&event=displaySum"}],"Contributors":["Eric A. Bushong","Maryann E. Martone","Mark H. Ellisman"],"PUBMED":["PMID:15036382"]},"BIOLOGICALPROCESS":{"onto_name":"forebrain astrocyte development","onto_id":"GO:0021897"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of 3 week old rat reveals highly ramified spongiform processes that span territories with minimal overlap. Astrocytes were filled with Lucifer Yellow (green) and Alexa 568 (red)  Optical sections were generated with a single photon confocal microscope."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"cell projection cytoplasm","onto_id":"GO:0032838"},{"onto_name":"cell body","onto_id":"GO:0044297"}],"RELATIONTOINTACTCELL":{"onto_name":"vibratome-sectioned tissue","onto_id":"FBbi:00000030"},"SOURCEOFCONTRAST":{"onto_name":"intracellular filling","onto_id":"FBbi:00000088"},"GROUP_ID":"10473","TECHNICALDETAILS":{"free_text":"Male Sprague Dawley rats, 3 weeks of age, were anaesthetized and perfused transcardially with with Ringer\u2019s solution (37°C), followed by 4% paraformaldehyde (PFA) in PBS (37°C, pH 7.4).  Tissue was sectioned (100 µm thick) using a vibratome, and injected  with a BX50WI microscope (Olympus, Melville, NY) and, using infrared differential interference contrast videomicroscopy,cells with a small diameter soma (approx. 7\u201311µm) in the stratum\nradiatum of CA1 were located and impaled with glass micropipettes filled with either 5% aq. LY, 20mM AlexaFluor 568 in 200mM KCl, or 20mM AlexaFluor 488 in 200mM KCl. The astrocytes were filled using a 0.5 Hz pulses of\ncurrent until all of the processes were brightly fluorescent. After several astrocytes were filled, a slice was placed in the 4% PFA overnight at 4°C. Slices were then imaged after coverslipping in Gelvatol.  Confocal images were acquired with a BioRad Radiance2000\n(Hercules, CA) attached to a Nikon E600FN\n(Kanagawa, Japan) microscope equipped with a Nikon (Tokyo, Japan) 60× oil objective (NA 1.4).  Optical z resolution, 0.15 µm. Additional details are provided in Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86."},"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":1052,"Pixel_size":{"unit":"microns","value":0.030833},"axis":"X"}},{"Space":{"Image_size":1052,"Pixel_size":{"unit":"microns","value":0.030833},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Rattus","onto_id":"NCBITaxon:10114"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"lucifer yellow","onto_id":"FBbi:00000057"},{"onto_name":"Alexa Fluor 568","onto_id":"FBbi:00000442"}],"CELLTYPE":{"onto_name":"astrocyte","onto_id":"CL:0000127"}}},"Citation":{"Title":"Eric A. Bushong, Maryann E. Martone, Mark H. Ellisman (2011) CIL:36300, Rattus, astrocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil36300","DOI":"doi:10.7295\/W9CIL36300"}}}