{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303099200},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"13372.tif","Size":26000000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"13372.zip","Size":25982048,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"13372.jpg","Size":19007,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Journal of Cell Biology","Href":"http:\/\/jcb.rupress.org\/content\/179\/2\/187.long"}],"PUBLISHED":["JCB vol. 179 no. 2 187-197"],"Contributors":["Iain M. Porter","Sarah E. McClelland","Guennadi A. Khoudoli","Christopher J. Hunter","Jens S. Andersen","Andrew D. McAinsh","J. Julian Blow","Jason R. Swedlow"],"PUBMED":["17938248"]},"BIOLOGICALPROCESS":[{"onto_name":"mitosis","onto_id":"GO:0007067"},{"free_text":"Bod1 depletion"}],"PROCESSINGHISTORY":{"onto_name":"constrained iterative deconvolution","onto_id":"FBbi:00000360"},"CELLLINE":{"onto_name":"HeLa S3","onto_id":"MCC:0000220"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Z focal series of Bod-1 depleted HeLa cell at mitotic metaphase stained for DNA (blue), Aurora B (green) and tubulin (red).  Bod1 is a protein that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis.  This image is the Bod-1 depletion experiment for the control CIL: 13374."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"chromosome, centromeric region","onto_id":"GO:0000775"},{"free_text":"aurora B"},{"onto_name":"spindle","onto_id":"GO:0005819"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"11195","TECHNICALDETAILS":{"free_text":"Cells were fixed with 3.7% PFA or in methanol for 2 min at −20°C.  Mouse anti\u2013α-tubulin DM1A (Sigma-Aldrich), and mouse anti\u2013Aurora B antibody AIM-1 (BD Biosciences) were used at 1:500. Fluorescently labeled secondary antibodies were all obtained from Jackson ImmunoResearch Laboratories.  Image corresponds to Figure 5A from JCB vol. 179 no. 2 187-197."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":371,"Pixel_size":{"unit":"microns","value":0.0663},"axis":"X"}},{"Space":{"Image_size":353,"Pixel_size":{"unit":"microns","value":0.0663},"axis":"Y"}},{"Space":{"Image_size":33,"Pixel_size":{"unit":"microns","value":0.2},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"chemically fixed tissue","onto_id":"FBbi:00000002"},"VISUALIZATIONMETHODS":{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"}}},"Citation":{"Title":"Iain M. Porter, Sarah E. McClelland, Guennadi A. Khoudoli, Christopher J. Hunter, Jens S. Andersen, Andrew D. McAinsh, J. Julian Blow, Jason R. Swedlow (2011) CIL:13372, Homo sapiens. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13372","DOI":"doi:10.7295\/W9CIL13372"}}}