{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1286078400},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"7324.jpg","Size":2120,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"7324.tif","Size":2300000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"7324.zip","Size":2202968,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Clarke, Margaret","Silverman, Philip"],"PUBMED":["PMID: 19004777"]},"BIOLOGICALPROCESS":[{"onto_name":"pilus assembly","onto_id":"GO:0009297"},{"onto_name":"pilus retraction","onto_id":"GO:0043108"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"This is one of four related images of F-pili (conjugative pili encoded by the F plasmid of E. coli) dynamics by live-cell imaging.  There are 2 movie (.avi) files and 2 files (.tiff) from the movies.  The movies show that F-pili normally undergo cycles of extension and retraction in the absence of any obvious triggering event such as contact with a recipient cell.\n\nRetraction of an F-pilus. E. coli strain HfrH expressing the red fluorescent marker DsRed Express were mixed with R17 bacteriophage labeled with Alexa 488 (green); R17 bacteriophage (NCBITaxon:12026) bind to the sides of F-pili. \n\nSamples were imaged by using a Zeiss LSM 510 confocal microscope with a Plan-Apochromat 63 × 1.4 N.A. DIC objective. Alexa-488 was excited with the 488-nm line of an argon laser with a 505\u2013530-nm filter for emission, and DsRed was excited with the 543-nm line of a HeNe laser, with a 560-nm long pass filter for emission. An HFT UV\/488\/543 beam splitter was used.  Images were collected in a single focal plane at 2 s intervals and accompanying movies (.avi) are playing at 10fps."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"pilus","onto_id":"GO:0009289"},"RELATIONTOINTACTCELL":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"SOURCEOFCONTRAST":[{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"},{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"}],"GROUP_ID":"1456","MOLECULARFUNCTION":{"onto_name":"conjugation","onto_id":"GO:0000746"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":116,"Pixel_size":{"unit":"microns","value":0.114},"axis":"X"}},{"Space":{"Image_size":100,"Pixel_size":{"unit":"microns","value":0.114},"axis":"Y"}},{},{"Wavelength":{"unit":"nanometers","value":"488, 532"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Escherichia coli","onto_id":"NCBITaxon:562"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"DsRed-ExpressFP","onto_id":"FBbi:00000506"},{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"macromolecular probe","onto_id":"FBbi:00000410"}],"CELLTYPE":{"onto_name":"prokaryotic cell","onto_id":"CL:0000520"}}},"Citation":{"Title":"Clarke, Margaret, Silverman, Philip (2010) CIL:7324, Escherichia coli, prokaryotic cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil7324","DOI":"doi:10.7295\/W9CIL7324"},"Microbial_type":["bacteria"]}}