{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1309233600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"35377.jpg","Size":3879459,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"35377.zip","Size":26929258,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"35377.tif","Size":27000000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Hans Ris"]},"BIOLOGICALPROCESS":{"onto_name":"nuclear division","onto_id":"GO:0000280"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"HeLa","onto_id":"MCC:0000219"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Whole human female metaphase chromosome from metaphase arrested cell swollen in hypotonic medium, and treated to remove histones. The residual 'scaffold' was imaged with high voltage EM at 1 MeV.  This image was taken with a specimen tilt of 54 degrees. Grouped with it is an image of the same area at 46 degrees tilt, providing an oblique stereo view of the chromosome."},"ITEMTYPE":{"onto_name":"film","onto_id":"FBbi:00000303"},"CELLULARCOMPONENT":{"onto_name":"condensed chromosome","onto_id":"GO:0000793"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"8653","TECHNICALDETAILS":{"free_text":"HeLa cells were metaphase-arrested by a 3hr treatment with colcemid, the cells mechanically broken by passage through a 22-gauge needle and exposed to hypotonic 75 mM KCl, treated to remove histones, spread on a water surface, fixed with formaldehyde, picked up on  formvar coated grid, critical point dried, and imaged with the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic microscopy of chromosomes. Meth Cell Biol 22:77-96."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_id":"FBbi:00000622"},"DIMENSION":[{"Space":{"Image_size":5770,"Pixel_size":{"unit":"microns","value":1.5},"axis":"X"}},{"Space":{"Image_size":2333,"Pixel_size":{"unit":"microns","value":1.5},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"critical_point dried specimen","onto_id":"FBbi:00000581"}],"VISUALIZATIONMETHODS":[{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"}],"CELLTYPE":{"free_text":"cevical carcinoma"}}},"Citation":{"Title":"Hans Ris (2011) CIL:35377, Homo sapiens, cevical carcinoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35377","DOI":"doi:10.7295\/W9CIL35377"}}}