{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1330059600},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"26564.zip","Size":4802314,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"26564.tif","Size":4900000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"26564.jpg","Size":10569,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Journal of Cell Biology","Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/3072\/6734\/"}],"PUBLISHED":["P Sinclair et al. 2010 Dynamic plasticity of large scale chromatin structure revealed by self-assembly of engineered chromosome regions. J Cell Biol 109:761-776"],"Contributors":["Paul Sinclair","Qian Bian","Matt Plutz","Edith Heard","Andrew Belmont"],"PUBMED":["20819934"]},"BIOLOGICALPROCESS":[{"onto_name":"nucleus organization","onto_id":"GO:0006997"},{"onto_name":"chromatin organization","onto_id":"GO:0006325"}],"PROCESSINGHISTORY":{"onto_name":"constrained iterative deconvolution","onto_id":"FBbi:00000360"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Image shows an immuno-FISH z-series of a mouse ES cell carrying a multi-copy insertion of alpha-globin BAC showing partial co-localization of lac operator FISH signal (green) with H3K9me3 immunostaining (red).  DAPI staining is blue."},"ITEMTYPE":{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},"CELLULARCOMPONENT":[{"onto_name":"heterochromatin","onto_id":"GO:0000792"},{"onto_name":"nuclear chromatin","onto_id":"GO:0000790"},{"free_text":"transgene"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"}],"GROUP_ID":"10842","TECHNICALDETAILS":{"free_text":"Formaldehyde fixed cells were imaged using an IMT-2 Olympus fluorescence microscope with 60x 1.4 NA objective lens. Z-series were recorded, and the slices processed by iterative constrained deconvolution.   \nSee also: P Sinclair et al. 2010 Dynamic plasticity of large scale chromatin structure revealed by self-assembly of engineered chromosome regions. J Cell Biol 109:761-776."},"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":180,"Pixel_size":{"unit":"microns","value":0.067},"axis":"X"}},{"Space":{"Image_size":233,"Pixel_size":{"unit":"microns","value":0.067},"axis":"X"}},{"Space":{"Image_size":19,"Pixel_size":{"unit":"microns","value":0.2},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"4',6-diamidino-2-phenylindole (DAPI)","onto_id":"FBbi:00000056"},{"onto_name":"EGFP","onto_id":"FBbi:00000082"}],"CELLTYPE":{"onto_name":"embryonic stem cell","onto_id":"CL:0002322"}}},"Citation":{"Title":"Paul Sinclair, Qian Bian, Matt Plutz, Edith Heard, Andrew Belmont (2012) CIL:26564, Mus musculus, embryonic stem cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil26564","DOI":"doi:10.7295\/W9CIL26564"}}}