{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1306900800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"13746.zip","Size":792002,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"13746.tif","Size":800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13746.jpg","Size":33093,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/3512"}],"PUBLISHED":["J Cell Biol. 2010. 191: 943-952."],"Contributors":["Tiffiney R. Hartman","Daniel Zinshteyn","Heather K. Schofield","Emmanuelle Nicolas","Ami Okada","Alana M. O'Reilly"],"PUBMED":["21098113"]},"BIOLOGICALPROCESS":[{"onto_name":"germ-line stem cell division","onto_id":"GO:0042078"},{"onto_name":"ovarian follicle cell development","onto_id":"GO:0030707"},{"onto_name":"positive regulation of smoothened signaling pathway","onto_id":"GO:0045880"},{"onto_name":"positive regulation of hh target transcription factor activity","onto_id":"GO:0007228"},{"onto_name":"smoothened signaling pathway","onto_id":"GO:0007224"},{"onto_name":"cell adhesion","onto_id":"GO:0007155"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Boi (green) and Hedgehog (Hh, red) are expressed in apical cells of the Drosophila wild-type germarium (CIL# 13744). In the absence of boi (boi[e] mutant), Hh is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13745). Expression of Boi in apical cells in the boi[e] mutant (boi[e]; UAS-Boi\/+; babGal4\/+) rescues Hh localization to apical cells (this image). However, expression of a Boi mutant lacking the Hh-binding domain does not (CIL# 13747). Nuclei are labeled (DRAQ5, blue). Image is Fig 3C in J Cell Biol. 2010. 191: 943-952. Images in Fig 3 include CIL# 13744, 13745, 13746, 13747, 13748."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"extracellular region","onto_id":"GO:0005576"},{"onto_name":"cytoplasmic membrane-bounded vesicle","onto_id":"GO:0016023"},{"onto_name":"endocytic vesicle","onto_id":"GO:0030139"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"}],"GROUP_ID":"8382","MOLECULARFUNCTION":[{"onto_name":"protein binding","onto_id":"GO:0005515"},{"onto_name":"morphogen activity","onto_id":"GO:0016015"},{"onto_name":"patched binding","onto_id":"GO:0005113"},{"onto_name":"cysteine-type endopeptidase activity","onto_id":"GO:0004197"}],"TECHNICALDETAILS":{"free_text":"Fly ovaries were dissected and fixed as described previously (O\u2019Reilly et al., 2008). For anti-Boi immunostaining, ovaries were fixed in 2% formaldehyde on ice. For nuclear staining, fixed ovaries were incubated for 15 min with Draq5 (Cell Signaling Technology). Primary antibodies were 1:50 rat anti-Boi (Hartman et al., 2010); 1:100 goat anti-Hh (Santa Cruz Biotechnology, Inc.) or 1:100 rabbit anti-Hh (Ballet et al. 2003). Secondary antibodies used were FITC and Cy3 conjugated to species-specific secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.). Samples were mounted in Vectashield mounting medium. Images were collected at room temperature (22C) using 40× (1.25 NA) or 63× (1.4 NA) oil immersion lenses (Leica) on an upright microscope (DM 5000; Leica) coupled to a confocal laser scanner (TCS SP5; Leica). LAS AF SP5 software (Leica) was used for data acquisition. Images representing individual channels of single confocal slices from the center of each germarium were exported as TIFF files, and images were converted to figures using Photoshop software (Adobe)."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.1991},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.1991},"axis":"Y"}},{"Space":{"Image_size":1,"Pixel_size":{"unit":"microns","value":0.1998},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":[{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"},{"onto_name":"Cy3","onto_id":"FBbi:00000449"},{"onto_name":"membrane-permeant probe","onto_id":"FBbi:00000421"},{"free_text":"DRAQ5"}],"CELLTYPE":[{"onto_name":"germ line cell","onto_id":"CL:0000039"},{"onto_name":"follicle cell","onto_id":"CL:0000477"},{"onto_name":"germ line stem cell","onto_id":"CL:0000014"},{"onto_name":"follicle stem cell","onto_id":"CL:0000441"}]}},"Citation":{"Title":"Tiffiney R. Hartman, Daniel Zinshteyn, Heather K. Schofield, Emmanuelle Nicolas, Ami Okada, Alana M. O'Reilly (2011) CIL:13746, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13746","DOI":"doi:10.7295\/W9CIL13746"}}}