{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1298437200},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"12216.zip","Size":50405168,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"12216.tif","Size":50500000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"12216.jpg","Size":46350,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/2014"}],"PUBLISHED":["Xu et al. 2009 J Cell Biol 187, 637-653"],"Contributors":["Z. Xu","H. Ogawa","P. Vagnarelli","J.H. Bergmann","D.F. Hudson","S. Ruchard","T. Fukagawa","W.C. Earnshaw","K. Samejima"],"PUBMED":["19951914"]},"BIOLOGICALPROCESS":{"onto_name":"mitosis","onto_id":"GO:0007067"},"PROCESSINGHISTORY":{"onto_name":"constrained iterative deconvolution","onto_id":"FBbi:00000360"},"CELLLINE":{"onto_name":"DT40","onto_id":"MCC:0000139"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Cell of the DT40 chicken B lymphocyte line  at mitosis. Z-series of widefield immunofluorescence images captured using a 100x\/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase in INCENP-OFF cells expressing TrAP-INCENP W766G. Histone H3 modification H3-S10ph shown in green. DAPI (DNA) is blue. See Supplementary Fig 3F panels 5-7 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653."},"ITEMTYPE":{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},"CELLULARCOMPONENT":[{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"},{"onto_name":"spindle","onto_id":"GO:0005819"},{"onto_name":"chromosome, centromeric region","onto_id":"GO:0000775"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"4132","DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"widefield illumination","onto_id":"FBbi:00000277"},"DIMENSION":[{"Space":{"Image_size":512,"axis":"X"}},{"Space":{"Image_size":512,"axis":"Y"}},{"Space":{"Image_size":32,"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Gallus gallus gallus","onto_id":"NCBITaxon:208526"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"},{"onto_name":"TexasRed","onto_id":"FBbi:00000453"},{"onto_name":"4',6-diamidino-2-phenylindole (DAPI)","onto_id":"FBbi:00000056"}],"CELLTYPE":{"onto_name":"memory B cell","onto_id":"CL:0000787"}}},"Citation":{"Title":"Z. Xu, H. Ogawa, P. Vagnarelli, J.H. Bergmann, D.F. Hudson, S. Ruchard, T. Fukagawa, W.C. Earnshaw, K. Samejima (2011) CIL:12216, Gallus gallus gallus, memory B cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil12216","DOI":"doi:10.7295\/W9CIL12216"}}}