{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1290920400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"7336.tif","Size":31800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"7336.jpg","Size":6162756,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"7336.zip","Size":31694336,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen (University of Hawaii)"]},"BIOLOGICALPROCESS":[{"onto_name":"plasma membrane to endosome transport","onto_id":"GO:0048227"},{"onto_name":"clathrin coat assembly","onto_id":"GO:0048268"},{"onto_name":"cytoskeletal anchoring at plasma membrane","onto_id":"GO:0007016"},{"free_text":"cytoskeletal organization"},{"onto_name":"plasma membrane organization","onto_id":"GO:0007009"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"}],"CELLLINE":{"free_text":"Cambridge Culture Collection, Cambridge, England, # LB1657\/1"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"A high resolution image of the pellicle of Opercularia coarctata. Indentations of the plasma membrane penetrate the alveolar membranes end as coated pits. These pits are also known as pellicular pores are homologous to parasomal sacs of other ciliates. The surface ridges contain an electron opaque rod in the cytosol of unknown composition, seen here in cross section. TEM taken on 6\/11\/69 by R. Allen with Philips 300 operating at 60kV. Neg. 30,000X. The raw film was scanned with a Epson Perfection V750 Pro. This image is best used for quantitative analysis.\n\nStandard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.\n\nAdditional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"clathrin coat of coated pit","onto_id":"GO:0030132"},{"onto_name":"plasma membrane organization","onto_id":"GO:0007009"},{"free_text":"alveolar membranes"},{"free_text":"rods of unknown material"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"7236","MOLECULARFUNCTION":{"onto_name":"clathrin binding","onto_id":"GO:0030276"},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":3623,"Pixel_size":{"unit":"nanometers","value":0.67},"axis":"X"}},{"Space":{"Image_size":4374,"Pixel_size":{"unit":"nanometers","value":0.67},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Opercularia [NCBITaxon:168247]","onto_id":"NCBITaxon:168247"},{"free_text":"Opercularia coarctata"}],"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2010) CIL:7336, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil7336","DOI":"doi:10.7295\/W9CIL7336"}}}