Neuron-targeted caveolin-1 improves motor function and preserves memory in mice subjected to brain trauma

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Studies in vitro and in vivo demonstrate that membrane/ lipid rafts (MLR) and caveolin (Cav) organize pro-growth receptors, and when over-expressed specifically in neurons, Cav-1 augments neuronal signaling and growth, and improves cognitive function in adult and aged mice. However, whether Cav-1 overexpression can preserve motor and cognitive function in the setting of brain trauma is unknown. Here, we engineered a neuron-targeted Cav-1 overexpressing transgenic (Tg) mouse (via synapsin promoter, SynCav1 Tg) and subjected it to a controlled cortical impact (CCI) model of brain trauma and measured biochemical, anatomical, and behavioral changes. SynCav1 Tg mice exhibited increased hippocampal expression of Cav- 1 and MLR-localization of PSF-95, NMDAR, and TrkB. When subjectd to CCI, SynCav1 Tg mice demonstrated preserved hippocampal-dependent fear learning and memory, motor function recovery on inverted grid, and decreased brain lesion volume. Conclusion: Neuron-targeted overexpression of Cav-1 in the adult brain preserves hippocampal- dependent learning and memory, restores motor function after brain trauam, and decreases brain lesion size. Our findings suggest neuron-targeted Cav-1 as a novel therapeutic strategy to restore nerve function and prevent trauma-associated neurodegeneration

• 2018

• Posadas, Edmund

1 online resource (23 unnumbered pages) : illustrations (black and white)

Description based on online resource; title PDF cover page (viewed May 23, 2019)

Forms part of the UC San Diego School of Medicine independent study projects, Class of 2018

Includes bibliographical references

Title provided by the cataloger

• Academic Dissertations

• Dissertations, Academic--School of Medicine. (Discipline)--UCSD

• text

• English

La Jolla, California: University of California, San Diego

M.D. University of California, San Diego 2018

• Posadas, Edmund

Under copyright (US)

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2020-10-27