{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1295240400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"12334.tif","Size":7100000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"12334.jpg","Size":1980162,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"12334.zip","Size":13740082,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/www5.pbrc.hawaii.edu\/allen\/"}],"Contributors":["Richard Allen"]},"BIOLOGICALPROCESS":[{"onto_name":"macronucleus organization","onto_id":"GO:0032124"},{"onto_name":"cytoplasm organization","onto_id":"GO:0007028"},{"free_text":"macronuclear replication"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop."}],"CELLLINE":{"free_text":"Euplotes sp. from Carolina Biological Supply"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"In Euplotes a replication band of the macronucleus has larger heterochromatin masses on one side of the band after DNA replication has occurred. The replication band moves in  the direction of lighter staining in the macronucleus. Vacuoles containing concretion bodies are observed. TEM taken on 8\/03\/67 by R. Allen with Philips 200  operating at 60kV. Neg. 7,200X. Bar = 1µm. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Another image (CIL:38871) is available for quantitative analysis. Additional information is available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"cytoplasm","onto_id":"GO:0005737"},{"onto_name":"macronucleus","onto_id":"GO:0031039"},{"onto_name":"food vacuole","onto_id":"GO:0020020"},{"onto_name":"contractile vacuole","onto_id":"GO:0000331"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"9827","DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":2341,"axis":"X"}},{"Space":{"Image_size":3000,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Euplotes sp.","onto_id":"NCBITaxon:178273"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (2011) CIL:12334, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil12334","DOI":"doi:10.7295\/W9CIL12334"}}}