{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303185600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"21573.tif","Size":5700000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"21573.zip","Size":11370522,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"21573.jpg","Size":2363711,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen (University of Hawaii)"]},"BIOLOGICALPROCESS":[{"onto_name":"cytoplasm organization","onto_id":"GO:0007028"},{"onto_name":"clathrin coat assembly","onto_id":"GO:0048268"},{"onto_name":"endosome organization","onto_id":"GO:0007032"}],"PROCESSINGHISTORY":[{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"},{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print of negative scanned for Photoshop"}],"PARAMETERIMAGED":[{"onto_name":"elastic scattering of electrons","onto_id":"FBbi:00000586"},{"onto_name":"electron density","onto_id":"FBbi:00000315"},{"onto_name":"elevation","onto_id":"FBbi:00000320"}],"IMAGEDESCRIPTION":{"free_text":"Vesicles and early endosomes under the membranelles of the peniculus of the buccal cavity. Cilia and basal bodies are tangentially fractured in this quick-freeze deep-etch preparation. Preendosomal vesicles, clathrin-coated and partially uncoated, that arise from the clathrin-coated ends of parasomal sacs lie nearest the basal body\/cilium complexes. Internal to these are the many early endosomes to which the uncoated preendosomal vesicles fuse and from which smaller clathrin-coated vesicles bud off. Blebs protrude from one early endosome. Carrier vesicles of about 100nm in diameter are found internal to the early endosomes. TEM taken on 7\/19\/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 12,000X. Bar = 0.5µm. Adapted with permission from J. Cell Sci. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:12636) is available for quantitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"cytoplasm","onto_id":"GO:0005737"},{"onto_name":"early endosome","onto_id":"GO:0005769"},{"onto_name":"clathrin coat of coated pit","onto_id":"GO:0030132"},{"onto_name":"vesicle membrane","onto_id":"GO:0012506"}],"RELATIONTOINTACTCELL":[{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"},{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"}],"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"GROUP_ID":"7148","DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"DIMENSION":[{"Space":{"Image_size":2658,"axis":"X"}},{"Space":{"Image_size":2141,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Paramecium multimicronucleatum","onto_id":"NCBITaxon:44030"},"PREPARATION":{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"},"VISUALIZATIONMETHODS":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2011) CIL:21573, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil21573","DOI":"doi:10.7295\/W9CIL21573"}}}