{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1326690000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39453.tif","Size":46700000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39453.jpg","Size":7133342,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"39453.zip","Size":46634482,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen (University of Hawaii)"]},"BIOLOGICALPROCESS":[{"onto_name":"macronucleus organization","onto_id":"GO:0032124"},{"onto_name":"digestive system process","onto_id":"GO:0022600"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"CELLLINE":{"free_text":"Cambridge Culture Collection, Cambridge, England, # LB1690\/1"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Only the macronucleus contains nucleoli which is the site of ribosomal RNA synthesis and ribosome subunit assembly. This EM also contains the distal end of the food vacuole forming cytopharynx which consists of a single membrane limited tube surrounded by microtubules that originate from the lamellae at the edge of the peristome. TEM taken on 3\/29\/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 10,250X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"macronucleus","onto_id":"GO:0031039"},{"onto_name":"nucleolus","onto_id":"GO:0005730"},{"onto_name":"microtubule associated complex","onto_id":"GO:0005875"},{"onto_name":"phagocytic cup","onto_id":"GO:0001891"},{"onto_name":"phagocytic vesicle membrane","onto_id":"GO:0030670"},{"free_text":"cytopharynx"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"10316","TECHNICALDETAILS":{"free_text":"Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":4566,"Pixel_size":{"unit":"nanometers","value":1.5},"axis":"X"}},{"Space":{"Image_size":5106,"Pixel_size":{"unit":"nanometers","value":1.5},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Vorticella convallaria","onto_id":"NCBITaxon:60850"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2012) CIL:39453, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39453","DOI":"doi:10.7295\/W9CIL39453"}}}