{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1290229200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"9717.zip","Size":8979538,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"9717.jpg","Size":2683222,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"9717.tif","Size":9000000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen"]},"BIOLOGICALPROCESS":[{"onto_name":"ciliary or flagellar motility","onto_id":"GO:0001539"},{"onto_name":"microtubule cytoskeleton organization","onto_id":"GO:0000226"},{"onto_name":"digestion","onto_id":"GO:0007586"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop."}],"CELLLINE":{"free_text":"Cambridge Culture Collection, Cambridge, England, catalog # LB1656\/1"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"A longitudinal section of somatic cilia of Coleps showing the axoneme, basal body, and attached fibrous and microtubular elements. Adjacent digestive vacuoles and cortical cytoplasm evident with typical ciliate tubular mitochondrial cristae. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5\/24\/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:2870) is available for quantitative analysis.\n\nAdditional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"cilium axoneme","onto_id":"GO:0035085"},{"onto_name":"axonemal microtubule","onto_id":"GO:0005879"},{"onto_name":"axonemal dynein complex","onto_id":"GO:0005858"},{"onto_name":"plasma membrane","onto_id":"GO:0005886"},{"onto_name":"microtubule basal body","onto_id":"GO:0005932"},{"onto_name":"cytoskeleton","onto_id":"GO:0005856"},{"onto_name":"microtubule cytoskeleton","onto_id":"GO:0015630"},{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"},{"onto_name":"food vacuole","onto_id":"GO:0020020"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"3259","MOLECULARFUNCTION":[{"onto_name":"structural molecule activity","onto_id":"GO:0005198"},{"free_text":"digestive activity"}],"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":2983,"axis":"X"}},{"Space":{"Image_size":3000,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Coleps hirtus","onto_id":"NCBITaxon:60000"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (2010) CIL:9717, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil9717","DOI":"doi:10.7295\/W9CIL9717"}}}