Microscopy product ID: 7769
2D Image
Description | Maximum intensity projection of a z series collected at a single time point of MDCK cells transfected with GFP-CX43 |
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Description | File containing original confocal imaging stack in Olympus Fluoview .oif format. An optical section series consisting of 21 optical sections was obtained at each of 41 time points (6 min intervals). |
Animation
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Description | Annotated time lapse animation of synchronized populations of MDCK cells expressing Cx43-GFP-4C imaged with confocal microscopy, revealing the dynamic redistribution of Cx43 during mitosis. Images were recorded every 6 min for a total duration of 4 h and 23 min. At each time point, 3D volume reconstructions from confocal image stacks over time are displayed (two frames per minute). The full resolution data are available for download. |
Reconstruction
Description | Maximum intensity projection of a confocal z series of MDCK cells expressing Cx43-GFP-4C imaged with confocal microscopy |
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Description | Full resolution time series with each time point representing a maximum intensity projection through the z series collected at 6 minute intervals in IMOD MRC format. |
Animation
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Description | Annotated time lapse animation of synchronized populations of MDCK cells expressing Cx43-GFP-4C imaged with confocal microscopy, revealing the dynamic redistribution of Cx43 during mitosis. Images were recorded every 6 min for a total duration of 4 h and 23 min. At each time point, 3D volume reconstructions from confocal image stacks over time are displayed (two frames per minute). The full resolution data are available for download. |
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- Cite This Work
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Boassa, Daniela; Sosinsky, Gina; Lampe, Paul; Solan, Joell (2017). Microscopy product ID: 7769. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0DB81NK
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
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Male
Product type: TIME SERIES. Microscopy type: LASER SCANNING CONFOCAL. Instrument: Olympus Fluoview 1000
- Funding
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NIH GM072881 and GM065937 awarded to Gina Sosinky, NIH GM55632 awarded to Paul Lampe
- Series
- Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Related Resources
- Boassa D, Solan JL, Papas A, Thornton P, Lampe PD, Sosinsky GE (2010). Trafficking and recycling of the connexin43 gap junction protein during mitosis. Traffic, 11(11):1471-1486. PubMed: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3272544/; Traffic. https://doi.org/10.1111/j.1600-0854.2010.01109.x
- Microscopy product 7769 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB7769
Primary associated publication
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- License
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Creative Commons Attribution 4.0 International Public License
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- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22