{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1328850000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39740.tif","Size":300000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39740.jpg","Size":22045,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"39740.zip","Size":272508,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Proceedings National Academy of Sciences USA","Href":"http:\/\/www.pnas.org\/content\/105\/26\/8872.long"}],"PUBLISHED":["A Routh et al. (2008) Nucleosome repeat length and linker histonen stoichiometry determine chromatin fiber structure. Proc Natl Acad Sci USA 105:8872-8877"],"Contributors":["Andrew Routh","Sara Sandin","Daniela Rhodes"],"PUBMED":["18583476"]},"BIOLOGICALPROCESS":[{"onto_name":"chromatin organization","onto_id":"GO:0006325"},{"onto_name":"cellular component organization or biogenesis","onto_id":"GO:0071840"}],"PROCESSINGHISTORY":{"onto_name":"montage","onto_id":"FBbi:00000261"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Images of negatively stained chromatin fibers prepared from tandem arrays of a nucleosome positioning sequence reconstituted with purified histones. These samples contained 61 repeats of a 197 bp DNA sequence reconstituted with core histones alone (leftmost image) plus different amounts of linker histone H5 (33%, 67%, and 100% respectively).\nSee: Fig 3A in Routh et al. (2008) Nucleosome repeat length and linker histonen stoichiometry determine chromatin fiber structure. Proc Natl Acad Sci USA 105:8872-8877."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"chromatin","onto_id":"GO:0000785"},{"onto_name":"nuclear chromatin","onto_id":"GO:0000790"}],"RELATIONTOINTACTCELL":{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"10708","TECHNICALDETAILS":{"free_text":"Reconstituted chromatin was fixed with 0.1% glutaraldehyde for 30 min, deposited on glow discharged carbon films, negatively stained with aqueous uranyl acetate, and examined with a Philips 2085 TEM operated at 80 KV.\nSee also: Routh et al. (2008) Nucleosome repeat length and linker histonen stoichiometry determine chromatin fiber structure. Proc Natl Acad Sci USA 105:8872-8877"},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":677,"axis":"X"}},{"Space":{"Image_size":134,"axis":"Y"}}],"PREPARATION":{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},"VISUALIZATIONMETHODS":[{"onto_name":"negative staining","onto_id":"FBbi:00000399"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"}]}},"Citation":{"Title":"Andrew Routh, Sara Sandin, Daniela Rhodes (2012) CIL:39740. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39740","DOI":"doi:10.7295\/W9CIL39740"}}}