{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1291006800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"7305.tif","Size":42000000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"7305.jpg","Size":9723555,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"7305.zip","Size":42105792,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen"]},"BIOLOGICALPROCESS":[{"onto_name":"water transport","onto_id":"GO:0006833"},{"free_text":"ionic transport"},{"onto_name":"micronucleus organization","onto_id":"GO:0032125"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},{"free_text":"raw unprocessed image"}],"CELLLINE":{"free_text":"Cambridge Culture Collection, Cambridge, England, # LB1657\/1"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"High resolution image of the contractile vacuole and the spongiome decorated tubules showing V-type proton pumps extending from the cytosolic side of the tubules; also detail of the micronucleus. TEM taken on 6\/10\/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis.\n\nStandard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.\n\nAdditional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"integral to contractile vacuolar membrane","onto_id":"GO:0031165"},{"onto_name":"smooth endoplasmic reticulum","onto_id":"GO:0005790"},{"free_text":"spongiome"},{"onto_name":"micronucleus","onto_id":"GO:0031040"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"3581","MOLECULARFUNCTION":[{"free_text":"v-type proton pumps"},{"onto_name":"proton motive force dependent protein transmembrane transporter activity","onto_id":"GO:0009977"}],"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":5576,"Pixel_size":{"unit":"nanometers","value":1},"axis":"X"}},{"Space":{"Image_size":3757,"Pixel_size":{"unit":"nanometers","value":1},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Opercularia [NCBITaxon:168247]","onto_id":"NCBITaxon:168247"},{"free_text":"Opercularia coarctata"}],"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (2010) CIL:7305, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil7305","DOI":"doi:10.7295\/W9CIL7305"}}}