{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1320379200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"38722.tif","Size":500000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"38722.jpg","Size":165187,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"38722.zip","Size":490470,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Journal of Cell Biology","Href":"http:\/\/jcb.rupress.org\/content\/48\/3\/594.full.pdf+html?sid=6c2cff91-8475-4246-8ec9-caaf88a6a9f5"}],"PUBLISHED":["UW Goodenough and LA Staehelin (1971) Structural differentiation of stacked and unstacked chloroplast membranes. J Cell Biol 48:595-619."],"Contributors":["Ursula W. Goodenough","L. Andrew Staehelin"],"PUBMED":["4396088"]},"BIOLOGICALPROCESS":[{"onto_name":"photosynthesis","onto_id":"GO:0015979"},{"onto_name":"plastid organization","onto_id":"GO:0009657"},{"onto_name":"chloroplast organization","onto_id":"GO:0009658"}],"CELLLINE":[{"free_text":"ac-5"},{"free_text":"ac-31"}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Isolated chloroplasts of the unicellular photosynthetic alga Chlamydomonas reinhardtii were cryofixed, freeze-fractured, and surface replicas observed by transmission electron microscopy. This close-up of wildtupe thylakoids shows the contrast in particle size and distribution between stacked and unstacked 'B' fracture planes, and unstacked 'C' planes (center). See Fig 6 in Goodenough and Staehelin 1971 J Cell Biol 48:595-619."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"chloroplast","onto_id":"GO:0009507"},{"onto_name":"plastid","onto_id":"GO:0009536"},{"onto_name":"plastid thylakoid membrane","onto_id":"GO:0055035"}],"RELATIONTOINTACTCELL":{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"},"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"GROUP_ID":"11387","TECHNICALDETAILS":{"free_text":"Isolated chhoroplasts were cryoprotected with glycerol, cryofixed, fractured at -100C, shadowed with Pt, and replicas prepared by carbon coating. Cleaned replicas were examined with a Philips 300 TEM. See Goodenough and Staehelin 1971 J Cell Biol 48:595-619"},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":746,"axis":"X"}},{"Space":{"Image_size":657,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Chlamydomonas reinhardtii","onto_id":"NCBITaxon:3055"},"PREPARATION":[{"onto_name":"cryofixed tissue","onto_id":"FBbi:00000013"},{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"}],"VISUALIZATIONMETHODS":{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"}}},"Citation":{"Title":"Ursula W. Goodenough, L. Andrew Staehelin (2011) CIL:38722, Chlamydomonas reinhardtii. CIL. Dataset","ARK":"ark:\/b7295\/w9cil38722","DOI":"doi:10.7295\/W9CIL38722"}}}