{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1317268800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"11940.zip","Size":22380672,"Mime_type":"application\/zip"},{"File_type":"Mp4","File_path":"11940_web.mp4","Size":2221818,"Mime_type":"video\/mp4"},{"File_type":"Jpeg","File_path":"11940.jpg","Size":37722,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["Inoue S 2008 Collected Works of Shinya Inoue World Scientific Publishing Co. Ltd. Hackensack, NJ pp 392-404."],"Contributors":["Shinya Inoue"]},"BIOLOGICALPROCESS":{"onto_name":"gastrulation","onto_id":"GO:0007369"},"PARAMETERIMAGED":{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"},"IMAGEDESCRIPTION":{"free_text":"A live developing sea urchin embryo was imaged using differential contrast microscopy to enhance cellular details.  Each minute a through focal series (Z-series) of micrographs was acquired to capture 3D information.  In the movie, several Z-series captured about 1 hr apart are presented, providing a '4D' view of this stage in embryo development."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"boundaries between regions with different refractive index","onto_id":"FBbi:00000599"},"TECHNICALDETAILS":{"free_text":"4-D images of a developing sea urchin gastrula (with DIC adjusted to show birefringence of developing spicules).  Every 60 seconds, through-focal optical sections were acquired in rapid sequence ( 5 sec\/stack of 104 frames) using stepper-motor-driven focusing controls on Shinya Scope 6, controlled by a  Universal Imaging MetaMorph program .  Out of the whole sequence, which lasted without interruption for 5 hours 20 minutes, four pairs of through-focal sequences separated approximately an hour apart are displayed here.\nSee Article 42 (S. Inoue and TD Inoue 1986. Ann NY Acad Sci 483:392-404) in Inoue S 2008 Collected Works of Shinya Inoue World Scientific Publishing Co. Ltd. Hackensack, NJ pp 392-404."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"differential interference contrast microscopy","onto_id":"FBbi:00000245"},"DIMENSION":[{"Space":{"Image_size":720,"axis":"X"}},{"Space":{"Image_size":480,"axis":"X"}},{"Space":{"Image_size":104,"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"free_text":"Sea urchin embryo"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"}}},"Citation":{"Title":"Shinya Inoue (2011) CIL:11940, Sea urchin embryo. CIL. Dataset","ARK":"ark:\/b7295\/w9cil11940","DOI":"doi:10.7295\/W9CIL11940"}}}