{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1280980800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"1435.zip","Size":23417234,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"1435.jpg","Size":4767359,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"1435.tif","Size":23300000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Ip, Wallace"]},"BIOLOGICALPROCESS":{"onto_name":"skeletal muscle contraction","onto_id":"GO:0003009"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":[{"onto_name":"electron density","onto_id":"FBbi:00000315"},{"onto_name":"elevation","onto_id":"FBbi:00000320"}],"IMAGEDESCRIPTION":{"free_text":"Rabbit psoas skeletal muscle fibers were blended in the presence of 5 mM MgATP to dissociate thick and thin filaments.  The medium was then gently replaced with 0.3M KCl to dissociate thick filaments.  Dissociated filaments were adsorbed onto mica flakes (Heuser JE, J Mol Biol. 1983 Sep 5;169(1):155-95), quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine.  The sample was then rotary-replicated with Pt-C at 11 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.\n\nImages were recorded on Kodak 4489 film at 75,000x magnification.  Stereo pairs were taken at +5 and -5 degrees.  Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"cytoskeleton","onto_id":"GO:0005856"},"RELATIONTOINTACTCELL":{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"},"SOURCEOFCONTRAST":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"MOLECULARFUNCTION":{"onto_name":"ATPase activity, coupled to movement of substances","onto_id":"GO:0043492"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"wide-field detection","onto_id":"FBbi:00000306"},{"onto_name":"widefield illumination","onto_id":"FBbi:00000277"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"},{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"}],"DIMENSION":[{"Space":{"Image_size":4149,"Pixel_size":{"unit":"microns","value":2.7E-4},"axis":"X"}},{"Space":{"Image_size":2797,"Pixel_size":{"unit":"microns","value":2.7E-4},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Oryctolagus cuniculus","onto_id":"NCBITaxon:9986"},"PREPARATION":[{"onto_name":"unfixed tissue","onto_id":"FBbi:00000014"},{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"},{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"}],"VISUALIZATIONMETHODS":{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},"CELLTYPE":{"onto_name":"skeletal muscle cell","onto_id":"CL:0000188"}}},"Citation":{"Title":"Ip, Wallace (2010) CIL:1435, Oryctolagus cuniculus, skeletal muscle cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil1435","DOI":"doi:10.7295\/W9CIL1435"}}}