{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1342497600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"40249.tif","Size":800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"40249.jpg","Size":65753,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"CCDB 7796","Href":"http:\/\/ccdb.ucsd.edu\/sand\/main?event=displayAll&mpid=7796"}],"Contributors":["Daniela Boassa"],"PUBMED":["20716111"]},"BIOLOGICALPROCESS":{"onto_name":"gap junction assembly","onto_id":"GO:0016264"},"CELLLINE":{"onto_name":"MDCK (NBL-2)","onto_id":"MCC:0000319"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"A single frame from a time-lapse animation of FlAsH-labeled MDCK cells expressing Cx43-4C309\/337 (green) showing their rearrangement and fate during and after mitosis.  In the time-lapse series, which can be accessed using the link provided to the Cell Centered Database, FlAsH fluorescence excitation z series along with a transmitted DIC image were recorded every 6 min for a total duration of about 5 h. 3D volume reconstructions from confocal image stacks over time are displayed at a rate of two frames per minute.  From Supplemental movie 2 from Boassa et al. (2010) Traffic. PMID 20716111."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"connexon complex","onto_id":"GO:0005922"},"RELATIONTOINTACTCELL":[{"onto_name":"living tissue","onto_id":"FBbi:00000025"},{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"}],"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"12252","MOLECULARFUNCTION":{"onto_name":"connexin binding","onto_id":"GO:0071253"},"TECHNICALDETAILS":{"free_text":"MDCK cells stably expressing two internal 4C domains in the C-terminus (FLNCCPGCCME)-tagged Cx43 (Cx43-4C309\/337) were labeled for 1 h at 37 degrees C with 180 nM FlAsH-EDT2\/12.5 uM EDT in Hanks balanced salt saline (HBSS). Free and non-specifically bound FlAsH was removed by washing with 2,3-dimercapto-1-propanol (BAL, 500 uM, 20min at 37 degrees C in HBSS). Time-lapse imaging was conducted using an Olympus FluoView1000 confocal microscope equipped with a temperature controlled chamber (at 37 degrees C) and a 60X 1.42 NA objective. Medium: Opti-MEM supplemented with 5% FBS covered with a glass coverslip."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":512,"axis":"X"}},{"Space":{"Image_size":512,"axis":"X"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Canis lupus familiaris","onto_id":"NCBITaxon:9615"},"PREPARATION":[{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"}],"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"},"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Daniela Boassa (2012) CIL:40249, Canis lupus familiaris, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil40249","DOI":"doi:10.7295\/W9CIL40249"}}}