{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1309233600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"35379.zip","Size":28617798,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"35379.jpg","Size":2733290,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"35379.tif","Size":28700000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Hans Ris"]},"BIOLOGICALPROCESS":[{"onto_name":"nuclear division","onto_id":"GO:0000280"},{"onto_name":"chromosome organization","onto_id":"GO:0051276"},{"onto_name":"mitosis","onto_id":"GO:0007067"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"HeLa","onto_id":"MCC:0000219"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"High Voltage transmission electron micrograph of human female (HeLa) mitotic chromosomes from metaphase arrested cell swollen in hypotonic medium and recorded at 1 MeV.  This image was taken with a specimen tilt of 55 degrees. Grouped with it is an image of the same area at 45 degrees tilt, providing an oblique stereo view of the chromosome."},"ITEMTYPE":{"onto_name":"film","onto_id":"FBbi:00000303"},"CELLULARCOMPONENT":[{"onto_name":"condensed chromosome","onto_id":"GO:0000793"},{"onto_name":"chromatin","onto_id":"GO:0000785"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"11178","TECHNICALDETAILS":{"free_text":"HeLa cells were metaphase-arrested by a 3hr treatment with colcemid, the cells mechanically broken by passage through a 22-gauge needle and exposed to hypotonic 75 mM KCl for 10 min. Samples were fixed with 3:1 methanol:acetic acid, squashed in 45% acetic acid, transferred to a formvar coated grid, critical point dried, and imaged with the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic microscopy of chromosomes. Meth Cell Biol 22:77-96."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_id":"FBbi:00000622"},"DIMENSION":[{"Space":{"Image_size":4296,"Pixel_size":{"unit":"microns","value":1.5},"axis":"X"}},{"Space":{"Image_size":3330,"Pixel_size":{"unit":"microns","value":1.5},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":[{"onto_name":"coagulating-fixative fixed tissue","onto_id":"FBbi:00000003"},{"onto_name":"critical_point dried specimen","onto_id":"FBbi:00000581"}],"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":{"free_text":"cevical carcinoma"}}},"Citation":{"Title":"Hans Ris (2011) CIL:35379, Homo sapiens, cevical carcinoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35379","DOI":"doi:10.7295\/W9CIL35379"}}}