{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1291006800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"9911.tif","Size":6700000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"9911.jpg","Size":1395283,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"9911.zip","Size":13279630,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen"]},"BIOLOGICALPROCESS":[{"onto_name":"water transport","onto_id":"GO:0006833"},{"free_text":"ionic transport"},{"onto_name":"micronucleus organization","onto_id":"GO:0032125"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop"}],"CELLLINE":{"free_text":"Cambridge Culture Collection, Cambridge, England, # LB1657\/1"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"An image of the contractile vacuole and the spongiome decorated tubules showing V-type proton pumps extending from the cytosolic side of the tubules; also detail of the micronucleus. TEM taken on 6\/10\/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. Bar = 0.25µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:7305) is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"integral to contractile vacuolar membrane","onto_id":"GO:0031165"},{"onto_name":"smooth endoplasmic reticulum","onto_id":"GO:0005790"},{"free_text":"spongiome"},{"onto_name":"micronucleus","onto_id":"GO:0031040"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"3581","MOLECULARFUNCTION":[{"free_text":"v-type proton pumps"},{"onto_name":"proton motive force dependent protein transmembrane transporter activity","onto_id":"GO:0009977"}],"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":3000,"axis":"X"}},{"Space":{"Image_size":2208,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Opercularia [NCBITaxon:168247]","onto_id":"NCBITaxon:168247"},{"free_text":"Opercularia coarctata"}],"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (2010) CIL:9911, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil9911","DOI":"doi:10.7295\/W9CIL9911"}}}