6023
2006-06-22 00:00:00.0
Kevin Knoops
purpose
Electron Tomography of Membrane Modification
120 kV
18500
Slow scan cooled 2K CCD camera
2008-01-04 00:00:00.0
6023
Zoom1
FEI Tecnai 12
TEM
MOSAIC
1.7 nm/pixels
2023
1.7 nm/pixels
2023
Marjolein Kikkert
Sjoerd H.E. van den Worm
Jessika C. Zevenhoven-Dobbe
Yvonne van der Meer
Leiden University Medical Center (LUMC, the Netherlands), Council for Chemical Sciences of the Netherlands Organization for Scientific Research (NOW-CW grants 700.52.306 and 700.55.002), European Commission under context of Euro-Asian SARS-DTV Network (SP22-CT-2004-511064)
Kèvin Knoops
Abraham J. Koster
A. Mieke Mommaas and Eric J. Snijder
2008-07-25 00:00:00.0
Membrane Modifications Induced by Corona Virus
2005-02-01 00:00:00.0
P2005
Knoops K, Kikkert M, Worm SH, Zevenhoven-Dobbe JC, van der Meer Y, Koster AJ, Mommaas AM, Snijder EJ. SARS-coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum. PLoS Biol. 2008 6(9):e226. PMID: 18798692
Mosaic electron micrograph covering the entire cross section of a VeroE6 cell infected with SARS-CoV. The image represents a 100nm thick plane through the center of the infected cell.
<![CDATA[http://ccdb.ucsd.edu/sand/main?event=rd&type=512v&mpid=6023]]>
Full sized version of the image in jpeg and zoomify format.
/telescience/home/CCDB_DATA_USER.portal/P2005/Experiment_6014/Subject_6016/Tissue_6018/Microscopy_6023/6023_zoom1.zip
18700,16000,59
.0017,.0017,.0017
VeroE6
kidney
For ultrastructural morphological investigations, SARS-CoV-infected Vero E6 cells were pre-fixed (for biosafety reasons) overnight with 3% paraformaldehyde in 0.1M PHEM buffer (60 mM piperazide-1,4-bis[2-ethanesulfonic acid], 25 mM HEPES, 2 mM MgCl2, 10 mM EGTA) at various time points after infection. For cryo-fixation, cell monolayers adhered to Thermanox coverslips (Nunc, Denmark) were plunged into liquid ethane. Freeze substitution was performed at -90C in an automated freeze-substitution system (Leica, Austria) using an FS medium consisting of 90% acetone and 10% water, containing 1% osmium tetroxide and 0.5 % uranyl acetate. After washing with pure acetone at RT, the samples were embedded in epoxy LX-12 resin. Thin sections were collected on 100 Mesh grids, 1.25% pioloform support film contrasted with uranyl acetate and lead hydroxide and subsequently viewed at 80 kV with a Philips CM-10 transmission electron microscope (Philips, The Netherlands).
time after infection
n/a
unspecified
Cercopithecus aethiops
monkey
Vervet
epithelium
Leica UC6 ultramicrotome
.1 um
7 hrs post-infection with SARS-CoV strain Frankfurt-1 (provided by Dr. H. F. Rabenau
603 and Dr. H. W. Doerr (Johann-Wolfgang-Goethe-Universität, Frankfurt am Main, Germany)