{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1351483200},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"43410.tif","Size":94500000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"43410.zip","Size":94383371,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"43410.jpg","Size":468026,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"PLoS article","Href":"http:\/\/www.plosone.org\/article\/info%3Adoi%2F10.1371%2Fjournal.pone.0044671"}],"PUBLISHED":["Zaritsky A, Natan S, Ben-Jacob E, Tsarfaty I (2012). Emergence of HGF\/SF -induced Coordinated Cellular Motility. PLOS ONE  7(9): e44671","assafzar@gmail.com"],"Contributors":["Assaf Zaritsky"]},"BIOLOGICALPROCESS":[{"free_text":"cell migration"},{"free_text":"wound healing"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"DA3"},"PARAMETERIMAGED":{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"},"IMAGEDESCRIPTION":{"free_text":"Wound Healing Assay\n    Time series DIC images of mouse DA3 cells, derived from the mouse mammary adenocarcinoma cell line D1-DMBA-3, induced in BALB\/C mice by dimethylbenzanthracene  were grown to 90% confluence. A scratch approximately 300um wide was made in the cell monolayer, and images were recorded every 14.5 min for 26 hr.\n    This time series image, obtained with no HGF\/SF  treatment, is part of a group of 17 time series images (CIL:43401 to CIL:43417) representing three different treatments: (1) no HGF\/SF (CIL:43406 to CIL:43411), (2) with HGF\/SF (CIL:43401 to CIL:43405), activating HGF\/SF-Met signaling, and (3) with PHA (Met inhibitor) and HGF\/SF (CIL:43412 to CIL:43417). The group also includes two movies (CIL:43418 and CIL:43419) created from the time series CIL:43402 and CIL:43406 respectively."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"boundaries between regions with different refractive index","onto_id":"FBbi:00000599"},"GROUP_ID":"17052","TECHNICALDETAILS":{"free_text":"DA3 cells expressing the fluorescent protein mCherry were grown to 90% confluence in wells of 2 cm diameter in DMEM supplemented with 10% heat-inactivated FCS (Gibco-BRL) and treated with or without the Met inhibitor PHA665752 (2.5 µM) for 2 hrs. A scratch was generated using a 200 µl tip, and the cells were incubated with or without 80 ng ml-1 Met-Hepatocyte Growth Factor\/Scatter Factor (HGF\/SF) and subjected to time lapse confocal laser scanning microscopy (CLSM-510, Zeiss, Germany) for approximately 26 hrs, with images taken at 14.5 min intervals. The position of each scratch was predefined, and a macro that repetitively positions the microscope on each point was executed. The acquired differential interference contrast (DIC) channel of the time-lapse sequence (shown here) was used for the multi-cellular analysis; the red fluorescence channel was exploited for single cell tracking.\nSee also: Zaritsky A, Natan S, Ben-Jacob E, Tsarfaty I (2012). Emergence of HGF\/SF -induced Coordinated Cellular Motility. PLOS ONE  7(9): e44671"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"onto_name":"differential interference contrast microscopy","onto_id":"FBbi:00000245"},"DIMENSION":[{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":1.24},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":1.24},"axis":"Y"}},{"Time":{"unit":"seconds","value":870,"frame":"77"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":{"free_text":"mammary adenocarcinoma"}}},"Citation":{"Title":"Assaf Zaritsky (2012) CIL:43410, Mus musculus, mammary adenocarcinoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil43410","DOI":"doi:10.7295\/W9CIL43410"}}}