{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1330318800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"40313.zip","Size":1205842,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"40313.tif","Size":1300000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"40313.jpg","Size":92392,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Proceedings National Academy of Sciences USA","Href":"http:\/\/www.pnas.org\/content\/100\/10\/5823.full.pdf+html?sid=400bc8e1-6feb-4a16-84a6-ae67ad1feeaa"}],"PUBLISHED":["J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827"],"Contributors":["Janet Cronshaw","Michael Matunis"],"PUBMED":["12730363"]},"BIOLOGICALPROCESS":[{"onto_name":"nucleus organization","onto_id":"GO:0006997"},{"onto_name":"nuclear transport","onto_id":"GO:0051169"},{"onto_name":"nuclear envelope organization","onto_id":"GO:0006998"}],"CELLLINE":{"onto_name":"HeLa","onto_id":"MCC:0000219"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Fluorescence images showing the distribution of GFP-ALADIN, a WD-repeat protein that is mutated in the human triple A syndrome (green), and Tpr, a nucleoporin that localizes to the nucleoplasmic filaments of nuclear pore complexes(red), within the nuclear envelope of HeLa cells. There is no overlap between Tpr and ALADIN.\nOther images in this group show the ultrastructural effects of mutant ALADIN."},"ITEMTYPE":{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},"CELLULARCOMPONENT":[{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"nuclear envelope","onto_id":"GO:0005635"},{"onto_name":"nuclear pore","onto_id":"GO:0005643"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"11064","TECHNICALDETAILS":{"free_text":"HeLa cells expressing GFP-ALADIN were fixed with formaldehyde immunostained for Tpr and observed with a Delta-vision deconvolution system.  \nSee: J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"wide-field detection","onto_id":"FBbi:00000306"},{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"}],"DIMENSION":[{"Space":{"Image_size":705,"axis":"X"}},{"Space":{"Image_size":570,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 594","onto_id":"FBbi:00000444"},{"onto_name":"EGFP","onto_id":"FBbi:00000082"}],"CELLTYPE":[{"onto_name":"epithelial cell","onto_id":"CL:0000066"},{"free_text":"cervical carcinoma"}]}},"Citation":{"Title":"Janet Cronshaw, Michael Matunis (2012) CIL:40313, Homo sapiens, epithelial cell, cervical carcinoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil40313","DOI":"doi:10.7295\/W9CIL40313"}}}