{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1291266000},"Data_type":{"Still_image":false,"Z_stack":false,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"9186.tif","Size":5800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"9186.jpg","Size":23814,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"9186.zip","Size":5846335,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Prabuddha Sengupta"]},"BIOLOGICALPROCESS":{"onto_name":"ER to Golgi vesicle-mediated transport","onto_id":"GO:0006888"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"HeLa","onto_id":"MCC:0000219"},"PARAMETERIMAGED":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"IMAGEDESCRIPTION":{"free_text":"Sialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment with Brefeldin A (BFA). \nImages were collected as a 4D time series (z-stack over time).  z-stacks of 48 individual timepoints are included in this collection of files as well as the movie SiEGFP_BFA.avi, which is the maximum projection of the z-stacks at the individual time points.\n\nBFA was added according to the following protocol:\nTimepoint1: Before addition of BFA 0seconds (8 Z-slices at 1 um apart)\nTimepoint2: After addition of BFA 130seconds (8 Z-slices at 1 um apart) \nTimepoint3: 238 seconds (11 Z-slices at 1 um apart) \nTimepoint3 thru Timepoint48: Images captured every 5 seconds (11 Z-slices at 1 um apart at each time point) \n\nImages were collected on a Carl Zeiss spinning disk confocal with a 63X 1.4 NA objective with 250 ms exposures at 5 s intervals."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"Golgi apparatus","onto_id":"GO:0005794"},{"onto_name":"endoplasmic reticulum","onto_id":"GO:0005783"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"7926","TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.11},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.11},"axis":"Y"}},{"Space":{"Image_size":8,"Pixel_size":{"unit":"microns","value":1},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"unfixed tissue","onto_id":"FBbi:00000014"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"},"CELLTYPE":[{"onto_name":"permanent cell line cell","onto_id":"CL:0000002"},{"onto_name":"epithelial cell","onto_id":"CL:0000066"}]}},"Citation":{"Title":"Prabuddha Sengupta (2010) CIL:9186, Homo sapiens, permanent cell line cell, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil9186","DOI":"doi:10.7295\/W9CIL9186"}}}