{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1312862400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"4614.tif","Size":2700000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"4614.zip","Size":1268048,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"4614.jpg","Size":58619,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Ginger Withers (Whitman College)","Dieter Brandner (Whitman College)"]},"BIOLOGICALPROCESS":[{"onto_name":"neuron development","onto_id":"GO:0048666"},{"onto_name":"dendrite development","onto_id":"GO:0016358"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Dendritic development in cultured hippocampal neurons after 17 days in vitro.  Neurons were immunostained for MAP2, a microtubule associated protein localized to dendrites but not axons to show the extent and complexity of the dendritic arbor at this relatively mature stage of development."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"cytoskeleton","onto_id":"GO:0005856"},{"onto_name":"microtubule cytoskeleton","onto_id":"GO:0015630"},{"onto_name":"dendrite","onto_id":"GO:0030425"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},"GROUP_ID":"9912","TECHNICALDETAILS":{"free_text":"Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc).  Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press).  Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained MAP2 (HM2, from Sigma with d549 conjugated secondary, excitation, 555, emission, 568, Jackson Immunoresearch).  Images were acquired with a Leica DMRA microscope with a 20X (Fluotar, NA 0.5) lens, Photometrics CoolSnap ES CCD camera and MetaMorph software."},"DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":1300,"Pixel_size":{"unit":"microns","value":0.339},"axis":"X"}},{"Space":{"Image_size":1030,"Pixel_size":{"unit":"microns","value":0.339},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Rattus","onto_id":"NCBITaxon:10114"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},"CELLTYPE":{"onto_name":"multipolar neuron","onto_id":"CL:0000104"}}},"Citation":{"Title":"Ginger Withers (Whitman College), Dieter Brandner (Whitman College) (2011) CIL:4614, Rattus, multipolar neuron. CIL. Dataset","ARK":"ark:\/b7295\/w9cil4614","DOI":"doi:10.7295\/W9CIL4614"}}}