{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1287028800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"754.zip","Size":20900084,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"754.jpg","Size":2677489,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"754.tif","Size":20700000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Rachel Horowitz-Scherer","Chris Woodcock"]},"BIOLOGICALPROCESS":{"onto_name":"fertilization","onto_id":"GO:0009566"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":[{"onto_name":"modes of electron microscopy","onto_id":"FBbi:00000256"},{"onto_name":"EELS imaging","onto_id":"FBbi:00000381"}],"IMAGEDESCRIPTION":{"free_text":"Phosphorus has a characteristic electron energy loss with an edge at 160 eV. The Zeiss 902 electron microscope permits images to be recorded at different energy losses which can be digitized and processed to yield a net phosphorus image.  For nuclear chromatin, phosphorus is primarily located in DNA, allowing its distribution in chromatin fibers to be mapped. Electron energy loss (EELS) imaging is also known as electron spectroscopic imaging (ESI) and electron energy filtering TEM (EFTEM).\nStarfish sperm chromatin, which has clearly defined chromatin fibers was fixed, embedded in Lowicryl at low temperature, thin sections prepared and imaged unstained. The 3 grouped images were recorded  with a Zeiss 902 energy filtered TEM at 80KV using  5eV windows centered on  energy loss values of 160 (this image), 120, and 104eV."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"nuclear chromatin","onto_id":"GO:0000790"},"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"inelastic scattering","onto_id":"FBbi:00000309"},"GROUP_ID":"8092","MOLECULARFUNCTION":{"free_text":"DNA packaging"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":3865,"Pixel_size":{"unit":"nanometers","value":0.4},"axis":"X"}},{"Space":{"Image_size":2804,"Pixel_size":{"unit":"nanometers","value":0.4},"axis":"X"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Patiria miniata","onto_id":"NCBITaxon:46514"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"free_text":"Low temperature embedding"}],"VISUALIZATIONMETHODS":{"free_text":"EELS window"},"CELLTYPE":{"onto_name":"sperm","onto_id":"CL:0000019"}}},"Citation":{"Title":"Rachel Horowitz-Scherer, Chris Woodcock (2010) CIL:754, Patiria miniata, sperm. CIL. Dataset","ARK":"ark:\/b7295\/w9cil754","DOI":"doi:10.7295\/W9CIL754"}}}