{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1307937600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"35278.jpg","Size":44482,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"35278.zip","Size":262620,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"35278.tif","Size":300000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"DATE":["10\/1993"],"Contributors":["Michael Cammer","Phyllis Novikoff"]},"BIOLOGICALPROCESS":{"onto_name":"interphase","onto_id":"GO:0051325"},"PROCESSINGHISTORY":{"onto_name":"3D-deconvolution","onto_id":"FBbi:00000357"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Primary rat hepatocytes in culture fixed and stained for beta-tubulin.  The microtubules form a network throughout the cytoplasm.  In this image they are seen originating from a brighter and denser region adjacent to the cell nucleus.\n\nThis is a single optical section a few microns up from the substrate collected by widefield fluorescence microscopy with a Photometrics camera with a KAF1400 chip.  The imaging was performed at a microscopy course at Woods Hole Oceanographic Institute in October 1993 and deconvolved on VayTec software running on a Macintosh computer.  At the time, deconvolution was new and novel for use by biologists."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"microtubule organizing center","onto_id":"GO:0005815"},{"onto_name":"cytoplasmic microtubule","onto_id":"GO:0005881"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"8536","TECHNICALDETAILS":{"free_text":"Image was collected with 1 60X, 63X, or 100X oil immersion objective with a numerical aperture of 1.25 such that each pixel size was 0.23 X 0.23 um.  The image was collected as part of a Z series with step size of 0.53 um.  A Photometrics cooled CCD camera based on the KAF1400 chip was used."},"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.232},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.232},"axis":"Y"}}],"MOUSE_GROSS_ANATOMY":[{"onto_name":"TS25,liver","onto_id":"EMAP:10869"}],"SPECIESTAXASPECIFIC":{"onto_id":"EMAP:EMAP:10869"},"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Rattus rattus","onto_id":"NCBITaxon:10117"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"}],"VISUALIZATIONMETHODS":[{"onto_name":"Rhodamine","onto_id":"FBbi:00000452"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"}],"CELLTYPE":{"onto_name":"hepatocyte","onto_id":"CL:0000182"}}},"Citation":{"Title":"Michael Cammer, Phyllis Novikoff (2011) CIL:35278, Rattus rattus, hepatocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35278","DOI":"doi:10.7295\/W9CIL35278"}}}