{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1409976000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"47320.zip","Size":3150502,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"47320.jpg","Size":107138,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"47320.tif","Size":3200000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Mark Harding"]},"BIOLOGICALPROCESS":{"onto_name":"cell death","onto_id":"GO:0008219"},"PROCESSINGHISTORY":{"free_text":"Converted from volocity to .avi"},"IMAGEDESCRIPTION":{"free_text":"Visualization of neutrophil recruitment in capillaries proximal to agarose beads. (Figure 4 - Propidium iodide experiments)"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"SOURCEOFCONTRAST":{"free_text":"antibody labelling"},"RELATIONTOINTACTCELL":{"free_text":"intact cells-in vivo imaging"},"GROUP_ID":"16612","TECHNICALDETAILS":{"free_text":"Mice were anesthetized with ketamine\/xylazine, and then subcutaneous tissue on a dorsal flap was exteriorized.  GFP staphylococcus aureus beads were inserted.  Antibodies were injected into the vasculature to label neutrophils (red) and endothelial cells (blue).  Blocking antibodies against the molecule Mac1 were administered after 2 hours of infection."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Pixel_size":{"unit":"microns","value":512},"axis":"X"}},{"Space":{"Pixel_size":{"unit":"microns","value":512},"axis":"Y"}}],"SPECIESTAXASPECIFIC":{"onto_id":":mouse (C57 Black 6)"},"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Staphylococcus aureus","onto_id":"NCBITaxon:1280"},"CELLTYPE":[{"onto_name":"neutrophil","onto_id":"CL:0000775"},{"onto_name":"endothelial cell","onto_id":"CL:0000115"}],"PATHOLOGICALPROCESS":{"free_text":":Infection with S. aureus beads in subcutaneous tissue"}}},"Citation":{"Title":"Mark Harding (2014) CIL:47320, Staphylococcus aureus, neutrophil, endothelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil47320","DOI":"doi:10.7295\/W9CIL47320"}}}