{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1338868800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"41365.tif","Size":100000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"41365.jpg","Size":11255,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"41365.zip","Size":19330,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Waltraud G. Muller","J. Bernard Haymann","Kunio Nagashima","Peter Guttman","Stephan Werner","Stefan Rehbein","Gerd Schneider","James G. McNally","Stefan Heim","Florian Mueller"]},"BIOLOGICALPROCESS":[{"onto_name":"nucleus organization","onto_id":"GO:0006997"},{"onto_name":"nucleolus organization","onto_id":"GO:0007000"}],"PARAMETERIMAGED":{"free_text":"x-ray scattering"},"IMAGEDESCRIPTION":{"free_text":"The image shows a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy.  Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material.  Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals.  The image represents a 15.6 nm thick slice of the computed tomogram in the x-y plane, and shows a nucleolus containing granular components of various size and density. The images have a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation), the latter corresponding to ~35 nm half-pitch resolution.  Additional X-ray images from the study are included in this CIL Group. \nSee also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"nucleolus","onto_id":"GO:0005730"}],"RELATIONTOINTACTCELL":{"free_text":"slice of 3D tomogram"},"SOURCEOFCONTRAST":{"onto_name":"intrinsic mass distribution","onto_id":"FBbi:00000607"},"GROUP_ID":"11959","TECHNICALDETAILS":{"free_text":"Mouse adenocarcinoma cells were grown on gold grids coated with cellulose nitrate, incubated with 270 nm diameter gold-coated silica beads, plunge frozen in liquid ethane, transferred to a Gatan 630 cryoholder, and imaged at -170C in the frozen hydrated state using the X-ray microscope at the Helmholz Zentrum, Berlin, Germany. The microscope uses partially coherent illumination and a zone plate objective, and allows the specimen to be observed over a range of tilts.  The images were aligned using the gold beads as fiduciary markers, and reconstructed using a reciprocal space algorithm. \nSee: G Schneider et al. 2010 Three-dimensional cellular ultrastructure resolved by X-ray microscopy. Nat Meth 7:985-987."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"X-ray micrograph","onto_id":"FBbi:00000260"},"DIMENSION":[{"Space":{"Image_size":138,"Pixel_size":{"unit":"nanometers","value":31.2},"axis":"X"}},{"Space":{"Image_size":138,"Pixel_size":{"unit":"nanometers","value":31.2},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"cryofixed tissue","onto_id":"FBbi:00000013"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":{"free_text":"adenocarcinoma"}}},"Citation":{"Title":"Waltraud G. Muller, J. Bernard Haymann, Kunio Nagashima, Peter Guttman, Stephan Werner, Stefan Rehbein, Gerd Schneider, James G. McNally, Stefan Heim, Florian Mueller (2012) CIL:41365, Mus musculus, adenocarcinoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil41365","DOI":"doi:10.7295\/W9CIL41365"}}}