{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1292821200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"10425.tif","Size":14100000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"10425.zip","Size":14360316,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"10425.jpg","Size":943087,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Hans Ris"]},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Nucleated erythrocytes from chicken were spread on water, the dispersed chromatin picked up on carbon-formvar grids, fixed with paraformaldehye, critical point dried and shadowed with platinum.  Images were obtained with the Wisconsin high voltage TEM at 1MEV using darkfield illumination.  For this micrograph, the grid was tilted to 44 degrees. A similar micrograph tilted to 55 degrees providing a stereo pair with an oblique 3D view of the tangled and irregular chromatin fibers is grouped with this image. The combination of shadowing and darkfield illumination gives a quasi-negative stain effect revealing nucleosomes in some fibers."},"ITEMTYPE":{"onto_name":"film","onto_id":"FBbi:00000303"},"CELLULARCOMPONENT":{"onto_name":"nuclear chromatin","onto_id":"GO:0000790"},"RELATIONTOINTACTCELL":{"free_text":"spread preparation"},"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"GROUP_ID":"2875","TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"darkfield illumination","onto_id":"FBbi:00000292"}],"DIMENSION":[{"Space":{"Image_size":2566,"Pixel_size":{"unit":"nanometers","value":0.5},"axis":"X"}},{"Space":{"Image_size":2742,"Pixel_size":{"unit":"nanometers","value":0.5},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Gallus gallus","onto_id":"NCBITaxon:9031"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"critical_point dried specimen","onto_id":"FBbi:00000581"}],"VISUALIZATIONMETHODS":{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},"CELLTYPE":{"onto_name":"erythrocyte","onto_id":"CL:0000232"}}},"Citation":{"Title":"Hans Ris (2010) CIL:10425, Gallus gallus, erythrocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil10425","DOI":"doi:10.7295\/W9CIL10425"}}}