{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1308888000},"Data_type":{"Still_image":false,"Z_stack":false,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"30564.zip","Size":231515621,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"30564.jpg","Size":42301,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"30564.tif","Size":231600000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"jcb-dataviewer","Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/1802\/"}],"PUBLISHED":["J Cell Biol. 2010 Jan 11;188(1):49-68."],"Contributors":["Gul Civelekoglu-Scholey","Li Tao","Ingrid Brust-Mascher","Roy Wollman","Jonathan M. Scholey"],"PUBMED":["PMID:20065089"]},"BIOLOGICALPROCESS":[{"onto_name":"regulation of mitotic prometaphase","onto_id":"GO:0035415"},{"onto_name":"regulation of microtubule-based process","onto_id":"GO:0032886"}],"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Dynamics of spindle microtubules and chromosomes during cycle 11 in a transgenic Drosophila embryo expressing GFP-tubulin (green) and histone-RFP (red). Only a single confocal plane containing the centrosomes was acquired during imaging. Images were acquired with exposure times of 1.8 s for GFP and 0.6 s for RFP, and total time shown is 432 s. This time series is the original data from Video 1 \"Dynamics of spindle MTs and chromosomes during cycle 11 in a transgenic Drosophila embryo expressing GFPtubulin and histone-RFP\" from Civelekoglu-Scholey et al.(2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope, J. Cell Biol. 188(1):49-68."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"spindle microtubule","onto_id":"GO:0005876"},{"onto_name":"kinesin complex","onto_id":"GO:0005871"},{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"8578","TECHNICALDETAILS":{"free_text":"Embryos expressing GFP-tubulin and histone-RFP were collected at 25°C for 1 h, matured for 40 min, dechorionated, placed on heptane glue and covered with halocarbon oil. Images from this image group were acquired with an inverted IX-70 Olympus with Ultra-View spinning disk confocal head (PerkinElmer) and acquired with an oil immersion objective (UPlan-Apochromat 100x N.A. 1.35, or Plan-Apochromat 60x NA 1.4).  Time series (at intervals of 3 to 10 sec) z-stacks of planes at 0.5 µm were acquired with an Orca II CCD camera (Hamamatsu Photonics).  For further information see J. Cell Biol. 188(1):49-68."},"DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":672,"axis":"X"}},{"Space":{"Image_size":512,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"Green fluorescent proteins from Aequorea","onto_id":"FBbi:00000437"},{"onto_name":"mRFP1","onto_id":"FBbi:00000527"}],"CELLTYPE":{"onto_name":"early embryonic cell","onto_id":"CL:0000007"}}},"Citation":{"Title":"Gul Civelekoglu-Scholey, Li Tao, Ingrid Brust-Mascher, Roy Wollman, Jonathan M. Scholey (2011) CIL:30564, Drosophila melanogaster, early embryonic cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil30564","DOI":"doi:10.7295\/W9CIL30564"}}}